FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_7_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_7_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40901130
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT663460.16221067730891542No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG507760.12414326939133467No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA498930.12198440483184696No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT479080.11713123818339494No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT477140.11665692365956637No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT473170.11568629032987596No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC450590.11016566045974768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA480150.025.5164664
GAGGTCA495300.022.5601232
TAATCCC543950.022.2721969
AGGTCAT513600.021.8223483
GTCATAA670800.018.4145955
CGAGGTC637200.017.2985551
ATAATCC823500.015.1573538
CATAATC825450.015.1215477
GTCCCAT534350.014.4278489
CGTCCCA548600.014.1186898
TCATAAT977850.012.9930286
TCGTCCC640250.012.5473647
TCTAGAC596850.012.1692517
CTAGACT663700.011.5244891
ATTCTAG718800.011.5066768
TTAAACC297900.011.38120657
CGTACAA779950.010.8998625
CATTCTA759150.010.8571497
GATATTA648800.010.6290044
TAGATAT581650.010.5969892
Produced by FastQC (version 0.11.5)