FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_56_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_56_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779414
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA94100.016.906014
TAATCCC118500.013.7286719
GAGGTCA110400.012.9923662
AGGTCAT114750.012.7344973
CTGGGAT97200.010.4557951
GTCATAA164000.010.0075885
CTAGACT142000.09.9488291
ATTCTAG162400.09.6186068
GGCGGAT131650.09.5264841
TCTAGAC131350.08.9877757
GGGAGCT60500.08.9353211
CCCGGGT54050.08.9347691
GTATGCC255250.08.754193140-144
GTCGGAT145650.08.70976451
CCGGGTT79150.08.6512121
CATTCTA192750.08.6269257
GGCAATC615300.08.557132140-144
GGCGGTT117750.08.4473871
CTCGGTT159000.08.3864631
CCGGGAT108500.08.3703781
Produced by FastQC (version 0.11.5)