FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_55_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_55_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502122
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA112750.022.664634
GAGGTCA123250.018.480142
AGGTCAT129950.017.3936313
TAATCCC147000.017.0411459
GTCATAA189300.014.1078285
CTAGACT185150.012.3796531
ATTCTAG201900.011.658658
CGAGGTC207050.011.3139231
GGGGCAT39850.010.6715811
ATAATCC261150.010.4193018
GACTTCA250150.010.4170534
GCGGGTT82950.010.3403611
CCCGGGT55250.010.1757741
CATAATC263000.010.1544177
CATTCTA235400.010.1218157
CCCGGAT71950.010.1180231
TCTAGAC169300.010.076937
GCCGGTT119450.09.9564121
GGCGGAT132400.09.9080571
TAGACTT239100.09.8877942
Produced by FastQC (version 0.11.5)