FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_54_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_54_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19760680
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA101900.018.8612024
AGGTCAT120050.014.3307213
GAGGTCA120100.014.28642
TAATCCC134400.014.0860379
GTCATAA177450.011.7234145
ATTCTAG176150.010.6248558
CTAGACT158700.010.4935861
TCTAGAC143250.010.2510387
CATTCTA207600.09.257987
GCGGGTT81900.09.2426021
GTATGCC318450.09.223006140-144
CCCGGAT69300.09.1545761
GTCGGTT138850.09.0342521
CCCGGGT54550.08.9867571
CGAGGTC197700.08.9705121
TAGCTTA723000.08.877392140-144
TATGCCG320050.08.70006140-144
GGCGGTT120350.08.6858191
GCCGGTT118750.08.681431
GTCGGAT151750.08.6463251
Produced by FastQC (version 0.11.5)