FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_53_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_53_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16805637
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA98550.017.749524
AGGTCAT112000.014.1397463
GAGGTCA113050.013.9656012
TAATCCC127650.013.646829
CTAGACT155700.011.2050251
TCTAGAC138000.011.1105997
GTCATAA167500.011.0447345
GCGGGTT72000.011.01402951
ATTCTAG166350.010.5585428
GACTTCA206400.09.41653254
AGACTTC202550.09.1690513
CCGGGTT65050.09.0876711
TAGACTT198950.09.0590392
CATTCTA198050.09.0502687
GTATGCC257050.08.76566140-144
GTCGGAT125850.08.76444151
CGAGGTC182150.08.7467991
GGCGGTT99400.08.63071251
GTCGGTT117000.08.5647561
CTGGGAT86900.08.5448311
Produced by FastQC (version 0.11.5)