FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_52_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_52_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17694364
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA89600.015.9875794
AGGTCAT106500.012.0987653
TAATCCC116400.011.9973799
GAGGTCA107100.011.9128552
CTAGACT132500.011.4244541
TCTAGAC123100.09.9409657
GTCATAA155000.09.8455925
GACTTCA192950.09.2894974
GGCGGAT117950.09.2280731
GTATGCC262200.09.142773140-144
TAGACTT176300.08.9950262
CTGGGAT92400.08.9713521
GGGAAAT136700.08.9114891
ATTCTAG153900.08.8401758
GTCGGTT126850.08.7510921
CTGGGTT47100.08.7234041
CATTCTA181500.08.6460454
AGACTTC188800.08.502383
TATGCCG262600.08.459946140-144
AGTGATC579550.08.439195140-144
Produced by FastQC (version 0.11.5)