FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_51_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_51_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17700836
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA89300.016.2024044
GAGGTCA100200.012.9513452
AGGTCAT104650.012.2437843
TAATCCC118350.011.9820979
ATTCTAG159350.011.4288718
CTAGACT147900.010.3342221
TCTAGAC132100.010.2989717
GCGGGTT77500.09.9538761
CATTCTA194450.09.587977
CCGGGTT72200.09.4862921
GTCGGTT123200.09.4216131
GTCATAA160550.09.3706745
GTCGGAT136750.09.3315981
GGCGGAT117650.09.1307081
TTCTAGA213050.08.8860569
CCCGGAT66150.08.8280591
ACTGACC744800.08.611908140-144
CTGACCA704450.08.473649140-144
GGGAAAT144050.08.4583091
GGGGCAT34400.08.3832351
Produced by FastQC (version 0.11.5)