FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_50_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_50_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182421
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA86800.015.5909164
AGGTCAT101000.012.2585913
GAGGTCA102500.012.2387982
TAATCCC112550.012.2157849
TCTAGAC114700.010.6688847
CTAGACT125650.010.6150641
GTCGGTT103100.010.5592161
GTCATAA144100.010.1406445
GGCGGAT99900.09.5984151
GCGGGTT61500.09.4956191
ATTCTAG139950.09.4640815
CCCGGGT38850.09.2788111
CTTAGGC516700.09.200853140-144
TAGGCAT443450.09.158998140-144
CATTCTA164500.09.1018764
AGGCATC429500.09.091091140-144
TTAGGCA485100.09.081962140-144
ACTTAGG547750.09.026252140-144
GTATGCC212600.08.993409140-144
GTCCCAT111750.08.8892499
Produced by FastQC (version 0.11.5)