FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_49_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_49_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20606659
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA122150.017.738354
GAGGTCA142850.014.2315212
AGGTCAT143250.014.1205593
TAATCCC163800.013.4476919
ATTCTAG217100.013.3292718
CTAGACT206700.011.7187471
CATTCTA252100.011.3930567
GTCATAA214500.010.8396545
TTCTAGA279950.010.5167869
TCTAGAC183100.010.2217487
GACTTCA268400.09.8697354
GTCGGAT152950.09.7095731
GTCGGTT148650.09.6509621
CCCGGAT76700.09.5870991
TAGACTT259000.09.5193822
CTCCTAT110600.09.3861991
CGAGGTC230400.09.261721
CCTTCAT329850.09.1222093
CCCGGTT72200.09.086291
GGCGGTT115500.09.0504131
Produced by FastQC (version 0.11.5)