FastQCFastQC Report
Thu 12 Nov 2020
HVMCCDRXX_l02_n01_bulk_48_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_48_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927017
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA3902.1827873E-1123.9991574
TCTAGCA3305.265151E-415.272198
GTCATAA7152.746674E-1015.1043635
AGGTCAT4903.1876098E-614.6933593
TAATCCC6451.742228E-814.5111179
CCGGAAT5205.837641E-613.859871
CCCTGCT3650.001121046613.8218981
CCGGGTT4252.2892989E-413.5663661
GGCGATT8303.529385E-812.1566091
CTGGGAT4755.8546476E-412.1383281
TGGAATC5952.3372068E-512.1128272
CCGGTTC4200.0032211612.0118882
GAAACTA6002.5733038E-511.99957754
CACGGAT4300.003841033411.7325421
GCCGTAT4958.278135E-411.6478911
CCCAATT6203.5611956E-511.6244081
GGGAGTT5009.005822E-411.5314111
GCCGGTT5059.788658E-411.417241
GACAGTA5802.475675E-411.1834822
GGCAGTA4550.00585465711.0878961
Produced by FastQC (version 0.11.5)