FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_48_run_3_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_48_run_3_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17002589
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA90300.018.2563484
GAGGTCA106150.014.6708582
AGGTCAT106100.013.8414283
TAATCCC122650.013.6758569
GTCATAA155250.011.8706495
CTAGACT137950.010.7140571
ATTCTAG149400.010.5044188
GGCGGTT100500.09.6130351
GGCGGAT120650.09.5612451
CCCGGTT62400.09.4743851
CTGGGAT88250.09.3134781
GACTTCA192650.09.2672054
GTCGGAT133250.09.252321
GGCGTAT97450.09.1000771
CATTCTA177050.09.0265797
GCCGGTT110550.08.9347751
ATAATCC211150.08.8984748
CCGGGCT78600.08.8975611
TAGACTT179600.08.8717172
CCCGGAT65000.08.7626511
Produced by FastQC (version 0.11.5)