FastQCFastQC Report
Thu 12 Nov 2020
HVMCCDRXX_l02_n01_bulk_47_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_47_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21771626
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA136950.024.6040424
GAGGTCA158900.019.6024482
AGGTCAT155100.019.5894973
TAATCCC181200.018.6750729
GTCATAA217850.015.9629155
CGAGGTC254450.012.2414191
ATAATCC296150.012.0827928
CTAGACT253350.011.8676731
CATAATC292750.011.7558397
ATTCTAG266000.011.5034998
GTCCCAT204050.011.2910949
CGTCCCA217900.010.8047118
GACTTCA325450.010.441924
CATTCTA298000.010.4373477
TAGACTT307450.010.2953342
GCGGGTT94850.09.95828151
AGACTTC319950.09.7438023
TCATAAT359350.09.7173256
GCCGGTT136050.09.4334781
CCCGGTT72450.09.2553971
Produced by FastQC (version 0.11.5)