FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_47_run_3_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_47_run_3_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21986451
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA139050.024.227994
GAGGTCA155500.019.6579062
AGGTCAT157200.019.2326553
TAATCCC182900.018.2619369
GTCATAA231000.015.0514275
CGAGGTC254400.012.1007571
ATTCTAG257000.011.9321588
CTAGACT246950.011.852741
ATAATCC301200.011.7824198
CATAATC303700.011.4484017
GTCCCAT202050.011.0088559
CGTCCCA210350.010.8140188
CATTCTA294500.010.461677
GCGGGTT94550.010.2937591
GACTTCA322400.010.0922024
TAGACTT299800.010.0278132
AGACTTC309100.09.7346383
GGCGGAT145950.09.5335571
TCATAAT378700.09.5232456
TTCTAGA328500.09.4884459
Produced by FastQC (version 0.11.5)