FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_35_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_35_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38705718
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA238600.019.5196234
TAATCCC299300.015.7533229
GAGGTCA271250.014.9373442
AGGTCAT279500.014.39685253
GTCATAA397000.012.3841945
GTCCCAT352400.09.8661749
CGAGGTC434100.09.5662011
TCTAGAC326800.09.5597217
CTAGACT351200.09.5045881
GGCGGAT261600.09.3701811
ATAATCC537300.09.3647868
CATAATC544600.09.2392587
ATTCTAG387650.08.9504488
CGTCCCA397300.08.859888
GCGGGTT175450.08.5881521
GGCGGTT228150.08.5635851
GTCACCC721650.08.1420145140-144
ACCCAAC608700.08.12485140-144
GTCGGAT290750.08.0588021
GGGGAAT154450.08.0287391
Produced by FastQC (version 0.11.5)