FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_34_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_34_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41036590
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA273500.016.4234924
AGGTCAT307500.013.132763
TAATCCC335600.013.10561859
GAGGTCA312350.012.971662
CTAGACT372050.010.948331
TCTAGAC328600.010.5807847
GTCATAA437350.010.4022245
ATTCTAG402900.09.4692778
GTCCCAT356950.09.09509859
GTCGGAT313200.08.747071
CCGGGTT163350.08.6945641
CGTCCCA372900.08.6867718
CATTCTA470050.08.6831494
GACTTCA499950.08.6821844
TAGACTT485850.08.6510162
ATAATCC572000.08.6079238
CGAGGTC486450.08.5958831
CATAATC575150.08.3354957
AGACTTC505150.08.2793083
CCAGTCA981800.08.081597140-144
Produced by FastQC (version 0.11.5)