FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_25_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_25_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34401946
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA286800.019.0502834
TAATCCC335850.016.1394399
GAGGTCA319700.015.7614282
AGGTCAT320100.015.6517323
GTCATAA426100.013.3292065
TCTAGAC337300.010.7347147
CATAATC534100.010.6473947
ATAATCC531150.010.6387678
CGAGGTC488300.010.3045661
CTAGACT370450.010.2356911
GTCCCAT342850.09.8680779
ATTCTAG382250.09.8678515
GTCGGAT262800.09.6281481
CGTCCCA360950.09.3931818
TAGATAT439300.09.3042812
GATATTA521150.09.2820814
GACTTCA477000.09.0395594
CATTCTA447950.08.9187154
TAGACTT456650.08.7297682
TCATAAT660300.08.7105256
Produced by FastQC (version 0.11.5)