FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_12_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_12_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38409874
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT625730.1629086312545571No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA530460.1381051132841519No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT472820.1230985553350162No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA440940.11479860621255877No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC439960.1145434634854569No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA431000.11221072998052531No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT430070.11196860473950006No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA427770.11136980037997522No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG423380.11022686510244735No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT413390.10762597138433726No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC389240.1013385256093264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA512000.021.9329974
TAATCCC559700.019.9222989
GAGGTCA532900.019.3179052
AGGTCAT553500.018.6108113
GTCATAA681450.016.7115145
CGAGGTC655000.015.4526631
CATAATC841500.013.7640187
ATAATCC837900.013.7028798
GTCCCAT477250.013.1677529
TAGATAT576150.013.1255482
GATATTA640200.012.6159664
CGTCCCA502150.012.5434768
TCTAGAC513100.012.3599657
CTAGACT575450.012.1768851
TTAAACC290850.011.9047247
TCATAAT988850.011.7712596
TCGTCCC571600.011.67437
TAAACCT280900.011.0707068
ATTATAG506250.010.9346435
CATTCTA668800.010.8386834
Produced by FastQC (version 0.11.5)