FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_11_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_11_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37014841
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT825780.22309429885164167No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT693420.18733566895505507No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG660340.17839871309996982No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA645230.17431656669820628No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC640570.1730576122155975No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA597290.16136500491789224No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT596310.16110024624987584No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT556050.150223527908711No Hit
TTTTATTATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATA479550.129556142089061No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC437730.11825797117431898No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA423240.11434332515436173No Hit
TATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAAT422430.11412449401038897No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA380610.10282632309564696No Hit
CTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAACAGA375920.10155926375585403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA634750.023.4408324
GAGGTCA642700.021.4470272
TAATCCC693050.021.4274229
AGGTCAT660300.020.8767433
GTCATAA811300.018.5438585
CGAGGTC753450.017.6821481
GTCCCAT574300.017.0089049
TAGATAT643150.016.7577782
CGTCCCA592650.016.4458248
GATATTA703650.016.1021294
ATTATAG557050.015.7264855
ATAATCC963550.015.7258138
TAAACCT299000.015.7208958
CATAATC968300.015.6189357
TTAAACC316250.015.3186257
TCGTCCC650050.015.2483367
TCATAAT1107250.013.7564146
CTCATTT440350.013.4082911
CGTACAA929850.012.641795
GTCTTTT839500.012.5892761
Produced by FastQC (version 0.11.5)