FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407556857
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA15609490.3830015305079262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG5464480.13407896115952234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5446480.1336373049907979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG25229400.0112.6338652
AAGCAGT25530600.0111.505691
CAGTGGT27835000.0101.8260654
AGTGGTA28391850.099.837145
GCAGTGG28490700.099.796833
GTGGTAT29403250.096.3413856
GGTATCA29767300.095.308058
TGGTATC30211550.093.635177
GTATCAA31596750.089.9615259
AGAGTAC29451000.025.94374515-19
ACATGGG28991800.025.72244820-24
CAGAGTA29923400.025.001215-19
TACATGG29231000.024.98211720-24
AACGCAG31866100.024.3803710-14
CAACGCA31525950.024.04752510-14
GCAGAGT30277950.023.73448215-19
GTACATG29936400.023.44334220-24
TCAACGC31662700.022.9317310-14
CGCAGAG30360100.020.87838415-19
ATCAACG31461650.020.26893610-14
Produced by FastQC (version 0.11.5)