FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n02_BS_10X_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n02_BS_10X_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21008317
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA940270.44757035987223537No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG318400.15155902302883187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1354500.0112.346852
AAGCAGT1358850.0112.345141
CAGTGGT1478750.0102.5272144
AGTGGTA1507000.0100.590915
GCAGTGG1519600.0100.126483
GTGGTAT1561800.096.994326
GGTATCA1585550.095.571628
TGGTATC1595850.094.84657
GTATCAA1696450.089.5743269
AGAGTAC1578550.026.00592615-19
ACATGGG1561450.025.72265620-24
TACATGG1571350.024.98327320-24
CAGAGTA1607600.024.97885115-19
AACGCAG1681600.024.7676210-14
CAACGCA1676200.024.23146810-14
GCAGAGT1614850.023.88673215-19
GTACATG1603800.023.5485220-24
TCAACGC1654700.023.52467310-14
CGCAGAG1628550.020.75114815-19
ATCAACG1686400.020.15030710-14
Produced by FastQC (version 0.11.5)