FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n02_BS_10X_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n02_BS_10X_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34470992
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA1104210.32033020691716674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG407290.11815441806838631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2370450.0113.575491
AGCAGTG2365600.0113.4957662
CAGTGGT2588400.0103.509464
GCAGTGG2631100.0102.045833
AGTGGTA2637700.0101.517495
GTGGTAT2722850.098.34336
GGTATCA2764250.096.8365868
TGGTATC2779700.096.1377647
GTATCAA2922400.091.8351069
AGAGTAC2750650.026.08007215-19
ACATGGG2697700.025.86603520-24
CAGAGTA2788250.025.16029515-19
TACATGG2722050.025.12893120-24
AACGCAG2932350.024.86622410-14
CAACGCA2915350.024.39184610-14
GCAGAGT2805150.024.06624815-19
TCAACGC2871650.023.75664710-14
GTACATG2787050.023.62425220-24
CGCAGAG2830700.021.1478715-19
ATCAACG2924900.020.58000610-14
Produced by FastQC (version 0.11.5)