FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407556857
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA1461750.088.161762
CTGGACG1073050.084.260211
ACGGAAG1075550.083.628841
CATCGGG1907000.082.406711
ACGGTCG1465350.080.012291
GGAAGCA993050.079.63233
GAACGGT1089550.078.2808153
GGGAGTA1499900.077.8809361
AGGAACG653300.077.2489248
GAAGGCC848300.074.739236
CGGGCAT1546150.074.72161
CCTATCG1637200.074.404511
ATCGACG1155000.074.119127
TCGACGA1119550.073.837138
CGGAAGG689850.073.270869
CGCAGGT1571100.073.1313251
AACGGGA1087200.072.862571
CCGTAGG1471900.072.7428051
TCACGGA1489400.071.547238
GGATCTA1708300.071.189651
Produced by FastQC (version 0.11.5)