FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n01_BS_10X_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n01_BS_10X_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21008317
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG84650.096.2671363
ATCGGGA85300.094.890332
CTGGACG64250.093.222151
AAGGTGC72850.091.306357
GAACGGT69000.090.349843
ACGGTCG79700.088.806431
TCGGGAG92350.087.616923
GAAGGCC53650.087.485576
AGGAACG41400.086.420078
CGGAAGG46600.086.354419
TCGGAAG47400.085.200688
AGGACGC59500.084.570488
CCTATCG92450.083.887371
CATCGGG99800.083.053611
AACGGGA65450.082.152421
GAGGCCT62250.081.628351
GTAGCGC73150.080.5987248
AAGGGAG52250.080.1853947
GCATGAC69400.080.07867
GCTCGTA64100.079.849124
Produced by FastQC (version 0.11.5)