FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l01_n01_BS_10X_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l01_n01_BS_10X_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34470992
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGCA185750.0117.311193
AAGGATG176650.0112.289142
GGGAGTA226250.0106.181711
GATGTAC181200.0105.398625
ACGGAAG92500.0104.493691
AAAGGAT264100.0104.173381
GTACTCC227500.0102.3959968
CATCGGC234600.0102.058716
GGATGTA193300.0102.003724
CACGACG189200.0100.942028
GCATCCG161050.099.71432
GATCCGA125800.099.6835256
AGGCATC173300.099.444663
AGCATCG117850.099.2001344
ACGCTTC242900.099.104783
GCAAAGC165000.098.64447
CGTGAAG108900.098.428917
GTAGCGA141250.098.412368
ATGCGAT231200.097.891461
GCGTACG88550.097.717376
Produced by FastQC (version 0.11.5)