FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_mb9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_mb9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23964456
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2439091.0177948541790391TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG793650.33117797458035353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT306250.063.79437642
CGTATGC322200.063.78588544
TCGTATG320500.063.75359743
TCTCGTA301300.063.47104641
ATGCCGT324650.063.16437547
TATGCCG325000.062.9777646
CGATCTC295150.062.87267338
TGCCGTC328250.062.5889248
GATCTCG297500.062.4585239
GCCGTCT329000.062.37138449
CACGATC298700.062.3483436
CCGTCTT329700.062.11131750
ACGATCT298450.062.0013237
TCACGAT316800.061.52602435
ATCTCGT302900.061.2986840
ATCACGA326550.061.14692334
CGTCTTC336850.061.00099651
CATCACG340650.060.78349733
CGTCTGA357450.059.83542616
ACGTCTG360700.059.14079315
Produced by FastQC (version 0.11.2)