FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_mb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_mb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18671310
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC202474610.84415608760178TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2433701.3034436255410038No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGC590520.3162713275072826TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC556880.2982543806513844TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC497510.26645693312359975TruSeq Adapter, Index 5 (98% over 50bp)
TATTAACCCTCACTAAAGGGAGCAGGACAGGCCAGAAAGTTCCCACTTAA467260.2502556060608495No Hit
TATTAACCCTCACTAAAGGGAGCCCATTAATTCTGCCATCTCTGAGTAAA436140.23358832347596392No Hit
TAATACGACTCACTATAGCCACAAAGCCAAGAGCCATCTGAGAAAAGAAG421060.22551176109228543No Hit
TATTAACCCTCACTAAAGGGAAGAGGGAGTGTCACGGTGTCTATAAATAA413330.22137171949906032No Hit
TAATACGACTCACTATAGCCGAGACTAGGAGAGACCAAGAATAACAGAAG387690.2076394211225672No Hit
TATTAACCCTCACTAAAGGGACCAGTTAAAGACGACCAACCCGCTCTCAA382660.20494544839114126No Hit
TAATACGACTCACTATAGCCAGGCAAGTGTCAGAGGAGATAACACACAAG374860.20076791612372138No Hit
TAATACGACTCACTATAGCCGTCCAGGTCACTTACTGCTCAGAATAAAAG371440.1989362288987757No Hit
TATTAACCCTCACTAAAGGGACACACAGAGAGTACCTGGGAACAAGGAAA368960.19760798787016015No Hit
TATTAACCCTCACTAAAGGGACAGAGAAGGCTCACCATACGAACTGTAAA364730.19534247998667476No Hit
TATTAACCCTCACTAAAGGGACCACTAACCACTCTGCCCAACTCCAAGAA357540.1914916521658095No Hit
TATTAACCCTCACTAAAGGGATGACACACCAGAGAGGGAAAGGATGCAAA354800.19002416006161324No Hit
TAATACGACTCACTATAGCCCACTTGTGGGAGTGTGTGGATTTGTTCAAG350680.18781756609471964No Hit
TATTAACCCTCACTAAAGGGAAACAAAGCAAACTCATCCGCTCATACCAA344230.18436306825819934No Hit
TAATACGACTCACTATAGCCTAAACAGTCAAGACGCACAACTCAAAAAAG320620.17171799943335525No Hit
TATTAACCCTCACTAAAGGGACGCTCGAAACTGTGGTTCAGGGATTGAAA319840.17130024620661324No Hit
TATTAACCCTCACTAAAGGGATAGCACAGCAAACTCACAAATTCACCAAA317160.16986488896601257No Hit
TAATACGACTCACTATAGCCAGCGACACGTTTAATATATTTATTCCCAAG316050.16927039398949512No Hit
TATTAACCCTCACTAAAGGGAGCTGGACTTGGCTAAGAAACAGTCATAAA315890.169184701019907No Hit
TAATACGACTCACTATAGCCTACCAGCTGGGCTTTGGGGCTGAAGAAAAG309150.16557488467600828No Hit
TAATACGACTCACTATAGCCACCTAAACCCTGGACTTGTCACTGTATAAG306790.16431091337458378No Hit
TAATACGACTCACTATAGCCGCTACCAGGTACAGAGACTCACTGTTCAAG300130.16074394351547908No Hit
TAATACGACTCACTATAGCCAACATCGAGACTTAGATACCGTTCCACAAG294490.15772326633749853No Hit
TAATACGACTCACTATAGCCACACGTCAGCTCATAGTAATGAAGAAAAAG292790.15681277853562497No Hit
TATTAACCCTCACTAAAGGGACATACTACGATAAACAGACAGATGCATAA289760.15518996792405032No Hit
TATTAACCCTCACTAAAGGGATAACAAGTCCGGTGGTCTGGGATGTGGAA288240.15437588471296337No Hit
TATTAACCCTCACTAAAGGGAGGATCAATGGGGTAAGTCTGGGAGTGAAA287070.15374925487285038No Hit
TAATACGACTCACTATAGCCGAAAGGAAGAGTGGTCGTTGGAAAATTAAG280180.15005910136996278No Hit
TATTAACCCTCACTAAAGGGAACATATACACTGGAGGTGCCAGAGATAAA279860.1498877154307866No Hit
TATTAACCCTCACTAAAGGGACGATGTTGGAAGAGTGACTGTGGGTCCAA278590.14920752748468105No Hit
TATTAACCCTCACTAAAGGGACAGGAGCAGATATGGGAGAGTCATTGAAA275710.14766505403209523No Hit
TATTAACCCTCACTAAAGGGATGCACACCCAGTAACATGACGTTTTGAAA272860.1461386480113072No Hit
TAATACGACTCACTATAGCCGAACCAGAGAGATGAAGGTACTCGTTCAAG272810.1461118689583109No Hit
TATTAACCCTCACTAAAGGGAGAGAGAAACTTAGCGGGGACCTTGATCAA270530.14489074414168046No Hit
TATTAACCCTCACTAAAGGGAGCAGAACCTTCTAAGACTATTGCATTAAA268720.14392134242321508No Hit
TAATACGACTCACTATAGCCACAAAGAACAAAGCTGGATTTGTTAAGAAG265270.14207358776647167No Hit
TAATACGACTCACTATAGCCCCAGCGAGTGTACCATAAACTCAAGAAAAG265060.14196111574388728No Hit
TATTAACCCTCACTAAAGGGACTGAGCAGTGTGAAGCAGACTCGAGTGAA264680.14175759494111556No Hit
TATTAACCCTCACTAAAGGGAGATCTGGAAAGGACATGTATGGTTTGGAA262940.14082568389684494No Hit
TAATACGACTCACTATAGCCCCGAGGACCTGTATTCTTGAGACAATAAAG262920.14081497227564643No Hit
TAATACGACTCACTATAGCCACCAGCGACAACCCAGACAAAAGATGGAAG261140.13986163798897883No Hit
TATTAACCCTCACTAAAGGGACAATTAATCAACACCAGGATTTGAAGTAA257950.13815313440781607No Hit
TATTAACCCTCACTAAAGGGAAGCCTACAGTTGGGTGTTTGAGAAGACAA257640.1379871042792391No Hit
TATTAACCCTCACTAAAGGGAGGTTGCCAATAGACTTGGTTGGTATTTAA257570.13794961360504432No Hit
TATTAACCCTCACTAAAGGGACAATGGAAAAGTCTCTGAGCAGTTTAGAA257190.13774609280227257No Hit
TAATACGACTCACTATAGCCACCCCAGCCAAGTCTAGCCCAAACAACAAG256390.13731762795433208No Hit
TATTAACCCTCACTAAAGGGAGGTGGATGGGCCTTTCCCATGATTTCAAA255080.1366160167658295No Hit
TATTAACCCTCACTAAAGGGAGGACTGAGCTCTTTAGGAACAATGAAAAA254720.13642320758425627No Hit
TATTAACCCTCACTAAAGGGACCATGGTACCTTGCAGACTAGGAAGGAAA252420.13519137114642732No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC251900.134912868995266TruSeq Adapter, Index 5 (98% over 50bp)
TATTAACCCTCACTAAAGGGAGTGTGAGCCCAAAGACTTATGTGTTGAAA250000.1338952649814073No Hit
TAATACGACTCACTATAGCCCAAAGCACTGTGATGTTTGTTGGAAGGAAG246070.13179043141589958No Hit
TATTAACCCTCACTAAAGGGAACCAACACAGGTCAAAATGTTCCAACAAA245560.13151728507533753No Hit
TATTAACCCTCACTAAAGGGACAGTGAGAAAAACCTCGTCTGGAAAGGAA243660.1304996810614788No Hit
TATTAACCCTCACTAAAGGGACCGTTCTTGAACTGGGCATACTGGTACAA241030.1290911028738744No Hit
TAATACGACTCACTATAGCCCCCTAACAAACCCCTAAGTGTGACCAGAAG238990.12799851751162614No Hit
TAATACGACTCACTATAGCCAGGAACTGAACCTTACTGGAACCTTTTAAG238970.1279878058904276No Hit
TATTAACCCTCACTAAAGGGACTAATCAGTGGTGAAATGATTGGATCGAA237830.1273772434821124No Hit
TAATACGACTCACTATAGCCGAGCTGGAGGAGCAGTCACAACACAGCAAG237570.12723799240653172No Hit
TATTAACCCTCACTAAAGGGAGCTCCCATGTCGTCCTCATTTTCTTGAAA237420.12715765524754288No Hit
TAATACGACTCACTATAGCCCAATACCAGAGCACACCATTTAACATGAAG237040.12695413444477116No Hit
TAATACGACTCACTATAGCCTTACTGCCCACCTACATCCATATTCCAAAG236610.12672383458900313No Hit
TATTAACCCTCACTAAAGGGACAAGCCCAAATCCTGTAGCTGCCTTTAAA235960.12637570690005145No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT235850.12631679298345963TruSeq Adapter, Index 5 (100% over 50bp)
TATTAACCCTCACTAAAGGGAGCTCCACAGTTGGCAGGTGATAACACGAA234880.12579727935533178No Hit
TATTAACCCTCACTAAAGGGAGGCTACCATGACTCTTGTCCCATCCCCAA234690.1256955189539459No Hit
TAATACGACTCACTATAGCCAAACAAAATCAAGGTAAAGCCTCACTTAAG234650.1256740957115489No Hit
TATTAACCCTCACTAAAGGGAAGAGGAAACCCAACCAGCTTCTCTGCAAA234080.1253688145073913No Hit
TAATACGACTCACTATAGCCACAGATCTTAGGACACCACATATCCCCAAG232800.12468327075068647No Hit
TATTAACCCTCACTAAAGGGACCGAGTGTAGGTGCAAGTCACAGGAGGAA231310.12388525497139728No Hit
TAATACGACTCACTATAGCCGGAAGCAGATAAATCGCACATCTCAAAAAG231220.12383705267600398No Hit
TATTAACCCTCACTAAAGGGACACCAGGTGAATCTTACTTCCACATACAA230030.12319971121469249No Hit
TATTAACCCTCACTAAAGGGAACTTGACTGGGCAAAAGTCTCAAGAGGAA229060.12268019758656463No Hit
TAATACGACTCACTATAGCCTCAATGACTGCACACCTTTCTCTGTAAAAG228970.12263199529117133No Hit
TATTAACCCTCACTAAAGGGAGCAGTTGTTCTAGTCGGTCTTACAAGCAA227870.12204285612525315No Hit
TAATACGACTCACTATAGCCTCTACACACAGTGCCTTTTGAGTAGTAAAG226210.12115379156577659No Hit
TATTAACCCTCACTAAAGGGAGCAGAGGTAGGTAGGAGAGTTGTCTAGAA224620.12030221768049483No Hit
TATTAACCCTCACTAAAGGGAACAAGACAGATCAGCAATTAGTCAACAAA223410.11965416459798481No Hit
TATTAACCCTCACTAAAGGGAGTTGAGCACCACTAATACGAAACTCTGAA223030.11945064379521309No Hit
TAATACGACTCACTATAGCCGCATGCCTATGTCTGTGCTTAGAAACTAAG222860.11935959501502573No Hit
TATTAACCCTCACTAAAGGGAATGAAAGGCAAAGATAAGGCAGCTTATAA221230.11848659788734694No Hit
TAATACGACTCACTATAGCCCAAACAACTACTGCAAACTAAATACAGAAG221190.11846517464494993No Hit
TATTAACCCTCACTAAAGGGACTGGTGGTTTAGACAACACGAGAGGAGAA220540.11811704695599827No Hit
TAATACGACTCACTATAGCCCAGAGAGCATACAAAAGGTAGTGCTTTAAG219700.11766715886566075No Hit
TATTAACCCTCACTAAAGGGACAGCTGGTCATGTCTTTTCCTGTGGTAAA219420.11751719616888157No Hit
TAATACGACTCACTATAGCCAGCAGCCACTGCTCCAGCAAACCTAAGAAG218820.1171958475329262No Hit
TAATACGACTCACTATAGCCTCAAAGCAAGCTCATGAAGTGGGATGTAAG218190.11685843146517302No Hit
TATTAACCCTCACTAAAGGGACCAATAGATGTGCAGTGTTCAGAGATTAA218140.11683165241217677No Hit
TAATACGACTCACTATAGCCCCACAACTCCTCACTCTTTGTCTTTGAAAG215240.11527846733839243No Hit
TATTAACCCTCACTAAAGGGAACTTGACACATCAACAGATCACTATTAAA214320.11478573276326084No Hit
TATTAACCCTCACTAAAGGGAGTGGGTAAGAGCTAACAAAGTTGGGACAA213770.11449116318030177No Hit
TAATACGACTCACTATAGCCGCAGGCTCCTCAGGCTCATGCATAAGTAAG213480.11433584467292332No Hit
TAATACGACTCACTATAGCCGATAAGGTTACTGATCCCGATAAAACAAAG213240.11420730521854118No Hit
TATTAACCCTCACTAAAGGGAGACGGCAGTGAGGCCACATTCATTTGCAA211190.11310936404569363No Hit
TAATACGACTCACTATAGCCCATGCCCAGCTTGGGGAATGTGAACACAAG210870.11293797810651744No Hit
TATTAACCCTCACTAAAGGGACCTTCCACCAATTAAGGGCTAGGATCCAA209280.11208640422123567No Hit
TATTAACCCTCACTAAAGGGAACTGTTGACAGCCTAAATATACCAAGAAA209080.11197928800925055No Hit
TAATACGACTCACTATAGCCATGCTGCAGCTAAGACAAGAGTGTTCGAAG208320.11157224640370707No Hit
TAATACGACTCACTATAGCCCAGGAGAGAGATTGCTAGGCTGTATGAAAG208070.11143835113872567No Hit
TATTAACCCTCACTAAAGGGAATGGAGCAAGAACCAAAGATCTGCTTGAA208050.11142763951752716No Hit
TAATACGACTCACTATAGCCTAGAGGGCCAAGGGTACAGGATCTTCAAAG207740.11126160938895022No Hit
TATTAACCCTCACTAAAGGGATCAAGCCAGCTATTGAACATTTCACAGAA207640.11120805128295765No Hit
TATTAACCCTCACTAAAGGGAGGTGTCACTTCCATTAAACAATTTCTAAA206040.11035112158707665No Hit
TATTAACCCTCACTAAAGGGATGGAAAAAGGATCAACTGGTCAGATTTAA204960.10977269404235696No Hit
TATTAACCCTCACTAAAGGGACTAAGTATGGGTAGAGGCCAGGAAATTAA204560.10955846161838671No Hit
TAATACGACTCACTATAGCCTGTGGAGGAGAGTGGTAGTTCAATTCAAAG203020.10873366678610125No Hit
TATTAACCCTCACTAAAGGGAACGGGAGAAGTGGAGCTGACGGGAGCCAA201770.1080641904611942No Hit
TATTAACCCTCACTAAAGGGACGGGACAGTGATGCTAGCAAATCCCTGAA201010.10765714885565073No Hit
TAATACGACTCACTATAGCCGGACCTTGAGGACACAGTCATAGCCTGAAG200500.10738400251508866No Hit
TAATACGACTCACTATAGCCGCCCTCAACTTTTAAACAACATCTTCCAAG200380.10731973278789758No Hit
TAATACGACTCACTATAGCCTGAGTGTTCTTGGCACCTTCAATTGGAAAG200280.10726617468190501No Hit
TAATACGACTCACTATAGCCAGTGCGACCAAAGACTTGAGACCAGGGAAG198840.1064949379556121No Hit
TAATACGACTCACTATAGCCGACCTACTCAGTGCCAACAGTCAACAGAAG198540.10633426363763443No Hit
TATTAACCCTCACTAAAGGGAGTAAGGTATATGTCTGGTGTATCTGGGAA198250.10617894513025598No Hit
TAATACGACTCACTATAGCCTCTGGGGACAGAACACGGTTCCTTTGAAAG197830.10595400108508723No Hit
TAATACGACTCACTATAGCCCACCTGAGACTTGGGCAGGAGTTACTAAAG197810.10594328946388872No Hit
TATTAACCCTCACTAAAGGGAAAAGTGAGCAGAGACTGTTTTGCTTAGAA197800.10593793365328945No Hit
TATTAACCCTCACTAAAGGGACCCGAGGTGGAGAGAGGAGAGTCCTGGAA196700.10534879448737125No Hit
TATTAACCCTCACTAAAGGGAAAGTGGGGAGAACCATTACTCGTGAGGAA196470.10522561084358836No Hit
TAATACGACTCACTATAGCCAGTATTGTGTTTACCCACGTGTTTGGCAAG195970.10495782031362556No Hit
TATTAACCCTCACTAAAGGGACAAAATGCGTGATAAACTGATGAAAGCAA194120.10396699535276314No Hit
TAATACGACTCACTATAGCCTGCTCTCAAGCAACTTCATATACAAGAAAG193640.10370991644399884No Hit
TAATACGACTCACTATAGCCGAACATGTCTGCTTGGCCATAATTTTAAAG193520.10364564671680777No Hit
TAATACGACTCACTATAGCCTATAACTAGCTACAGGGTGATAACATAAAG193220.10348497239883007No Hit
TATTAACCCTCACTAAAGGGATCTGAATGTATGACAAAAGTGCAACATAA192520.10311006565688213No Hit
TATTAACCCTCACTAAAGGGACAGAGAGTAATTAGCGAAAAGTAAGCCAA191450.10253699392276171No Hit
TAATACGACTCACTATAGCCATGCCAACTGGAAGCCTAGTACCATAGAAG190750.10216208718081378No Hit
TAATACGACTCACTATAGCCCAACACAATGAGAAAGACTATAATAGAAAG190690.10212995231721822No Hit
TAATACGACTCACTATAGCCGATAAGTCACACCCTGGCATAAAACATAAG190690.10212995231721822No Hit
TAATACGACTCACTATAGCCCGTCCAGTCACCAACCCAGGGATCCCGAAG189180.10132122491673054No Hit
TAATACGACTCACTATAGCCCTAAAGAAAGTGATAACCAAAGTGATTAAG188720.10107485762916475No Hit
TATTAACCCTCACTAAAGGGACAAGATGAGGGGGTGGATGTGAGAAAGAA187850.10060890210702945No Hit
TAATACGACTCACTATAGCCAGTATGGGAACATGGCTGCCATTAATAAAG187610.10048036265264729No Hit
TAATACGACTCACTATAGCCAGTGGAGTCTTTGAAAATAATATCCCAAAG187520.100432160357254No Hit
TATTAACCCTCACTAAAGGGACATATCGAGAACCACTTGATACAAGACAA187280.10030362090287184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAC7053150.069.760291
TAATACG6369050.069.6703951
ATACGAC6470700.068.6873553
TACGACT6473300.068.648244
AATACGA6478700.068.561662
ACGACTC6500000.068.53755
CACTATA6576450.068.52914411
TAGCCGT287900.068.45592516
CGACTCA6522550.068.422546
ATTAACC7194200.068.3689352
ACTATAG6593000.068.3661512
CTCACTA13854700.068.369
ATAGCCC1658850.068.324615
TATAGCC6580850.068.2668914
ACCCTCA7226300.068.224476
TCACTAT6603150.068.1953510
CTAAAGG7298350.068.0494713
GACTCAC6575450.068.042987
CTATAGC6609450.068.0417313
CCCTCAC7262000.068.014837
Produced by FastQC (version 0.11.2)