FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_mb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_mb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17971328
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC13921607.746561634176395TruSeq Adapter, Index 4 (100% over 50bp)
TATTAACCCTCACTAAAGGGAGCATTTGTTCCCATCTTTTCCAGGAGGAA2461861.369882069928277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2279331.2683147288836973No Hit
TAATACGACTCACTATAGCCAGTGCGACCAAAGACTTGAGACCAGGGAAG1233180.6861930292519285No Hit
TAATACGACTCACTATAGCCTCATGTCCTTTGGCTGTCTACTCAGAGAAG905420.5038136302447987No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC481040.2676708143104394TruSeq Adapter, Index 4 (98% over 50bp)
TAATACGACTCACTATAGCCCCCGGTGTCTGTTCAGTGTTGTTAGAGAAG457740.2547057179079921No Hit
TAATACGACTCACTATAGCCACAAAGCCAAGAGCCATCTGAGAAAAGAAG410000.22814118133061728No Hit
TAATACGACTCACTATAGCCGAGTGAGGAGAAAAGTGGCAATGGAAGAAG398480.22173097057713265No Hit
TAATACGACTCACTATAGCCATGCCAACTGGAAGCCTAGTACCATAGAAG389300.21662283388294953No Hit
TAATACGACTCACTATAGCCGTCCAGGTCACTTACTGCTCAGAATAAAAG369390.2055440755407725No Hit
TATTAACCCTCACTAAAGGGAGAGATGCCAGATTAGAAATCAGTCAGAAA353790.19686358181209537No Hit
TAATACGACTCACTATAGCCGAGACTAGGAGAGACCAAGAATAACAGAAG345940.19249551285247257No Hit
TAATACGACTCACTATAGCCTAAACAGTCAAGACGCACAACTCAAAAAAG332000.18473871268723158No Hit
TAATACGACTCACTATAGCCGGTAAAAACCTCCAAAACGATGAGCCAAAG327320.18213456456862842No Hit
TAATACGACTCACTATAGCCAGGCAAGTGTCAGAGGAGATAACACACAAG313380.17437776440338743No Hit
TATTAACCCTCACTAAAGGGAAGAGGGAGTGTCACGGTGTCTATAAATAA307000.17082766504512076No Hit
TATTAACCCTCACTAAAGGGAGCCCATTAATTCTGCCATCTCTGAGTAAA295090.16420044194841915No Hit
TATTAACCCTCACTAAAGGGAGAATTTGAGTTTGGCAAAAACAAATTGAA288420.160488974437504No Hit
TATTAACCCTCACTAAAGGGATAGCACAGCAAACTCACAAATTCACCAAA287540.1599993055605017No Hit
TATTAACCCTCACTAAAGGGACTCGGTGGAGTCTAGCCGGAATGCCAAAA284950.15855812102477904No Hit
TATTAACCCTCACTAAAGGGAGGACTGAGCTCTTTAGGAACAATGAAAAA281570.15667734738356565No Hit
TAATACGACTCACTATAGCCCAACAGGGTATTTCAATATATCTAGGTAAG281400.15658275225959928No Hit
TATTAACCCTCACTAAAGGGACACACAGAGAGTACCTGGGAACAAGGAAA278030.15470754303744275No Hit
TATTAACCCTCACTAAAGGGACATACTACGATAAACAGACAGATGCATAA276210.15369481876909707No Hit
TATTAACCCTCACTAAAGGGAGCAGGACAGGCCAGAAAGTTCCCACTTAA275750.15343885549248226No Hit
TATTAACCCTCACTAAAGGGAGGAGTGGGAATGTAATTCAGTGGTACAAA274590.15279338288188832No Hit
TAATACGACTCACTATAGCCACACGTCAGCTCATAGTAATGAAGAAAAAG273340.15209783049978276No Hit
TAATACGACTCACTATAGCCACCAGCGACAACCCAGACAAAAGATGGAAG273020.15191976908996374No Hit
TATTAACCCTCACTAAAGGGATTGGGTGCAGCCGAACAGAGAAGGGCAAA272960.15188638257562267No Hit
TATTAACCCTCACTAAAGGGATGACACACCAGAGAGGGAAAGGATGCAAA272740.1517639653563721No Hit
TAATACGACTCACTATAGCCAAACAAAATCAAGGTAAAGCCTCACTTAAG272400.1515747751084394No Hit
TATTAACCCTCACTAAAGGGAATCAGGAACAGCACTCTCGTAACAACGAA271630.1511463148410624No Hit
TATTAACCCTCACTAAAGGGATCTGATTGATCGATAACCGGAGACTCTAA270830.15070116131651484No Hit
TATTAACCCTCACTAAAGGGATGGAAAAAGGATCAACTGGTCAGATTTAA270420.1504730201351842No Hit
TATTAACCCTCACTAAAGGGACAGAGAAGGCTCACCATACGAACTGTAAA266560.14832515437924232No Hit
TAATACGACTCACTATAGCCCACTTGTGGGAGTGTGTGGATTTGTTCAAG265830.1479189517880927No Hit
TATTAACCCTCACTAAAGGGACCACTAACCACTCTGCCCAACTCCAAGAA265210.14757395780656832No Hit
TAATACGACTCACTATAGCCAACCAAGGATCATCTTTTGCTTAAATTAAG265000.1474571050063746No Hit
TATTAACCCTCACTAAAGGGAGGATCAATGGGGTAAGTCTGGGAGTGAAA264380.14711211102485025No Hit
TATTAACCCTCACTAAAGGGAGATCTGGAAAGGACATGTATGGTTTGGAA263590.14667252191935953No Hit
TAATACGACTCACTATAGCCGCTACCAGGTACAGAGACTCACTGTTCAAG263050.14637204329028994No Hit
TATTAACCCTCACTAAAGGGAGCTGGACTTGGCTAAGAAACAGTCATAAA260340.14486408572588516No Hit
TATTAACCCTCACTAAAGGGACTAATCAGTGGTGAAATGATTGGATCGAA257620.14335056374242347No Hit
TATTAACCCTCACTAAAGGGAGCAGAACCTTCTAAGACTATTGCATTAAA257200.14311685814203604No Hit
TAATACGACTCACTATAGCCTCTACACACAGTGCCTTTTGAGTAGTAAAG256870.14293323231316019No Hit
TATTAACCCTCACTAAAGGGACAATGGAAAAGTCTCTGAGCAGTTTAGAA251370.1398728018318958No Hit
TATTAACCCTCACTAAAGGGAGTCCAGAGTTATTATCGAGAAGACAGGAA249110.13861524312504897No Hit
TAATACGACTCACTATAGCCCCAGCGAGTGTACCATAAACTCAAGAAAAG248680.13837597310560468No Hit
TATTAACCCTCACTAAAGGGACAGGAGCAGATATGGGAGAGTCATTGAAA248320.13817565401955828No Hit
TAATACGACTCACTATAGCCTGAGTGTTCTTGGCACCTTCAATTGGAAAG247940.13796420609539817No Hit
TAATACGACTCACTATAGCCGAACCAGAGAGATGAAGGTACTCGTTCAAG247840.13790856190482975No Hit
TATTAACCCTCACTAAAGGGAGCTCCACAGTTGGCAGGTGATAACACGAA246630.13723526719895157No Hit
TAATACGACTCACTATAGCCCAAAGCACTGTGATGTTTGTTGGAAGGAAG246360.1370850278844168No Hit
TAATACGACTCACTATAGCCAACATCGAGACTTAGATACCGTTCCACAAG246110.13694591740799567No Hit
TAATACGACTCACTATAGCCTGCTCTCAAGCAACTTCATATACAAGAAAG245600.1366621320360966No Hit
TAATACGACTCACTATAGCCAGCGACACGTTTAATATATTTATTCCCAAG244300.13593875755870685No Hit
TAATACGACTCACTATAGCCACCAGAGCAATTTGTTTTTAGGAGTTGAAG243360.13541570216736348No Hit
TATTAACCCTCACTAAAGGGACTACTGTGGTTTAAAACATGATACCCAAA243290.1353767512339656No Hit
TATTAACCCTCACTAAAGGGACCGTTCTTGAACTGGGCATACTGGTACAA242470.13492046887130432No Hit
TATTAACCCTCACTAAAGGGAACCAACACAGGTCAAAATGTTCCAACAAA242330.1348425670045085No Hit
TAATACGACTCACTATAGCCGTGGCTTGACAACAGATGTTAAGTCCCAAG241920.1346144258231779No Hit
TAATACGACTCACTATAGCCCAAACAACTACTGCAAACTAAATACAGAAG237320.13205479305702952No Hit
TAATACGACTCACTATAGCCACATAATAGGTACTGTATAATAAATGAAAG237250.1320158421236316No Hit
TAATACGACTCACTATAGCCAGTGGAGTCTTTGAAAATAATATCCCAAAG235710.13115892158887757No Hit
TATTAACCCTCACTAAAGGGAATGAAAGGCAAAGATAAGGCAGCTTATAA235490.131036504369627No Hit
TATTAACCCTCACTAAAGGGAGTGGGTAAGAGCTAACAAAGTTGGGACAA235090.13081392760735322No Hit
TATTAACCCTCACTAAAGGGAAATCAGTCCAATTTCAGGTACACAATAAA232590.12942282284314216No Hit
TATTAACCCTCACTAAAGGGAAACAAAGCAAACTCATCCGCTCATACCAA232370.12930040562389156No Hit
TAATACGACTCACTATAGCCTCAATGACTGCACACCTTTCTCTGTAAAAG230420.12821534390780692No Hit
TAATACGACTCACTATAGCCCCCAAATGTTGTCTACCACTGTCGTTAAAG229680.12780357689760044No Hit
TATTAACCCTCACTAAAGGGAACTTGACACATCAACAGATCACTATTAAA228180.1269689140390738No Hit
TAATACGACTCACTATAGCCAGCCTTGCTATGTGCAGAAGGAGACTGAAG227860.1267908526292548No Hit
TATTAACCCTCACTAAAGGGAATGGAGCAAGAACCAAAGATCTGCTTGAA227500.1265905335432084No Hit
TATTAACCCTCACTAAAGGGAGTAAGGTATATGTCTGGTGTATCTGGGAA225850.12567240439882907No Hit
TAATACGACTCACTATAGCCGGAAGCAGATAAATCGCACATCTCAAAAAG225730.12560563137014694No Hit
TATTAACCCTCACTAAAGGGAGGTGTCACTTCCATTAAACAATTTCTAAA225250.1253385392554184No Hit
TATTAACCCTCACTAAAGGGAAGCCTACAGTTGGGTGTTTGAGAAGACAA224790.12508257597880357No Hit
TAATACGACTCACTATAGCCCCGAGGACCTGTATTCTTGAGACAATAAAG224770.12507144714068988No Hit
TATTAACCCTCACTAAAGGGACTGAGCAGTGTGAAGCAGACTCGAGTGAA224480.1249100789880414No Hit
TATTAACCCTCACTAAAGGGAAGAGATAGCAGAGCACACCAGAAGTTCAA224250.12478209734973397No Hit
TATTAACCCTCACTAAAGGGACAAGCCCAAATCCTGTAGCTGCCTTTAAA224000.12464298687331286No Hit
TAATACGACTCACTATAGCCTTACTGCCCACCTACATCCATATTCCAAAG223080.1241310603200832No Hit
TATTAACCCTCACTAAAGGGAGAGAGAAACTTAGCGGGGACCTTGATCAA222510.12381388843384306No Hit
TATTAACCCTCACTAAAGGGAAAGTGGGGAGAACCATTACTCGTGAGGAA221560.12328526862344284No Hit
TAATACGACTCACTATAGCCACCTAAACCCTGGACTTGTCACTGTATAAG220890.12291245254663428No Hit
TAATACGACTCACTATAGCCATGCTGCAGCTAAGACAAGAGTGTTCGAAG220440.12266205368907629No Hit
TAATACGACTCACTATAGCCGGGCCATATAGAATCCTACTACAGTAGAAG220360.12261753833662155No Hit
TAATACGACTCACTATAGCCTCCATAGCAATCATATTGCAAATCTGGAAG220000.12241721925057512No Hit
TATTAACCCTCACTAAAGGGACAATTAATCAACACCAGGATTTGAAGTAA219480.12212786945961923No Hit
TAATACGACTCACTATAGCCGAGCTGGAGGAGCAGTCACAACACAGCAAG218990.12185521292583386No Hit
TATTAACCCTCACTAAAGGGACGCTCGAAACTGTGGTTCAGGGATTGAAA218860.1217828754780949No Hit
TATTAACCCTCACTAAAGGGAAAAGTGAGCAGAGACTGTTTTGCTTAGAA218470.12156586313487795No Hit
TAATACGACTCACTATAGCCTTCGCACACTTGTCTCGAGCCTCTGCCAAG217420.1209815991339093No Hit
TATTAACCCTCACTAAAGGGATGCACACCCAGTAACATGACGTTTTGAAA216050.12021927372312163No Hit
TATTAACCCTCACTAAAGGGAGTGTGAGCCCAAAGACTTATGTGTTGAAA216040.12021370930406479No Hit
TAATACGACTCACTATAGCCTCTGGGGACAGAACACGGTTCCTTTGAAAG215260.11977968461763093No Hit
TATTAACCCTCACTAAAGGGACAGTGAGAAAAACCTCGTCTGGAAAGGAA214520.11936791760742443No Hit
TATTAACCCTCACTAAAGGGAACATATACACTGGAGGTGCCAGAGATAAA214030.11909526107363909No Hit
TATTAACCCTCACTAAAGGGAAGAGGAAACCCAACCAGCTTCTCTGCAAA213030.11853881916795465No Hit
TATTAACCCTCACTAAAGGGAGCAGAGGTAGGTAGGAGAGTTGTCTAGAA209840.11676376948882129No Hit
TAATACGACTCACTATAGCCGATAAGGTTACTGATCCCGATAAAACAAAG208390.11595692872557889No Hit
TAATACGACTCACTATAGCCCAACACAATGAGAAAGACTATAATAGAAAG207960.11571765870613457No Hit
TATTAACCCTCACTAAAGGGAACAAGACAGATCAGCAATTAGTCAACAAA207550.11548951752480396No Hit
TAATACGACTCACTATAGCCCACCTGAGACTTGGGCAGGAGTTACTAAAG207490.1154561310104629No Hit
TAATACGACTCACTATAGCCCTGCTAAAACACTATGACAAATCATAAAAG207250.11532258495309863No Hit
TATTAACCCTCACTAAAGGGACAGAGAGTAATTAGCGAAAAGTAAGCCAA206930.11514452354327961No Hit
TATTAACCCTCACTAAAGGGACTGCATAGCTGGTAATGCTGTTGTTAGAA205820.11452687302796988No Hit
TAATACGACTCACTATAGCCCAAGATGGTAGAGTCAGTGCACCAGGGAAG205050.11409841276059288No Hit
TAATACGACTCACTATAGCCGTCAGAGCTCCTCACGTAATGCAACCGAAG204870.11399825321756968No Hit
TATTAACCCTCACTAAAGGGATAAGAGGCTAAAGATACAAGGCTTAGGAA204040.1135364064358516No Hit
TAATACGACTCACTATAGCCTACACGTTACTTGTATCTTAAATGATAAAG203320.1131357682637588No Hit
TATTAACCCTCACTAAAGGGAGGTTGCCAATAGACTTGGTTGGTATTTAA201250.11198393351899204No Hit
TATTAACCCTCACTAAAGGGACACCAGGTGAATCTTACTTCCACATACAA198760.1105983931738378No Hit
TAATACGACTCACTATAGCCCAGACTCCAGAAATAGAACATATGCTAAAG198700.11056500665949673No Hit
TAATACGACTCACTATAGCCTCGACTCCATTCCAAAGCAGCGTTTCAAAG198690.11055944224043988No Hit
TATTAACCCTCACTAAAGGGATGAGGGAGATTTAAGAACTGTGGCAATAA198150.11025896361137029No Hit
TATTAACCCTCACTAAAGGGATCTGCACTCTGTGTTCAACTAGTCCTCAA197700.1100085647538123No Hit
TATTAACCCTCACTAAAGGGAGTTGAGCACCACTAATACGAAACTCTGAA197540.10991953404890278No Hit
TAATACGACTCACTATAGCCACCCCAGCCAAGTCTAGCCCAAACAACAAG197070.10965800635323109No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC196650.10942430075284364TruSeq Adapter, Index 4 (98% over 50bp)
TATTAACCCTCACTAAAGGGACTGGTGGTTTAGACAACACGAGAGGAGAA196180.10916277305717197No Hit
TAATACGACTCACTATAGCCGGACCTTGAGGACACAGTCATAGCCTGAAG195420.1087398772088518No Hit
TAATACGACTCACTATAGCCAGGAACTGAACCTTACTGGAACCTTTTAAG195240.10863971766582858No Hit
TATTAACCCTCACTAAAGGGATGCAAAGCATCAGATTTTGTCTAAGGAAA194220.10807214692203047No Hit
TAATACGACTCACTATAGCCGCCCTCAACTTTTAAACAACATCTTCCAAG194080.10799424505523465No Hit
TATTAACCCTCACTAAAGGGAGCAGTTGTTCTAGTCGGTCTTACAAGCAA193370.1075991713021987No Hit
TAATACGACTCACTATAGCCCTACCATACAGTTAGTAAGATCTTTAAAAG192880.10732651476841333No Hit
TAATACGACTCACTATAGCCCACTTAGGAGATAGCTGGAAAGATTGAAAG191740.10669217099593309No Hit
TAATACGACTCACTATAGCCATTGCAATGTGGAAAGTTGTAGCCCTAAAG191420.10651410958611406No Hit
TAATACGACTCACTATAGCCAGAAATGCTGAGTGTCAGAGACTATAGAAG191130.10635274143346557No Hit
TATTAACCCTCACTAAAGGGAGGTGGATGGGCCTTTCCCATGATTTCAAA190570.10604113396628229No Hit
TAATACGACTCACTATAGCCTGTGGAGGAGAGTGGTAGTTCAATTCAAAG190390.1059409744232591No Hit
TAATACGACTCACTATAGCCCGGCAGACGTCTTTGTTCTTTAGCAGAAAG190280.1058797658136338No Hit
TATTAACCCTCACTAAAGGGATGACAGATGGATGAATGATTGAATGTGAA189530.10546243438437049No Hit
TATTAACCCTCACTAAAGGGAAAGTGTACACATGTCTAAATAACCTTAAA189240.105301066231722No Hit
TAATACGACTCACTATAGCCTCCACTCTGGGACCCAGAGATTTGACCAAG189050.10519534226964196No Hit
TATTAACCCTCACTAAAGGGAGGCTACCATGACTCTTGTCCCATCCCCAA188550.10491712131679974No Hit
TATTAACCCTCACTAAAGGGAAAGCACTCAAAAGCTCTGGAAACATCAAA188450.1048614771262313No Hit
TAATACGACTCACTATAGCCTAGAGGGCCAAGGGTACAGGATCTTCAAAG188180.1047112378116965No Hit
TATTAACCCTCACTAAAGGGATTGTAAGCGCAGTGACAGAGTATGTATAA187780.10448866104942273No Hit
TAATACGACTCACTATAGCCACAGATCTTAGGACACCACATATCCCCAAG187760.10447753221130902No Hit
TAATACGACTCACTATAGCCACAAAGAACAAAGCTGGATTTGTTAAGAAG187090.10410471613450047No Hit
TATTAACCCTCACTAAAGGGACCTTCCACCAATTAAGGGCTAGGATCCAA186910.10400455659147725No Hit
TATTAACCCTCACTAAAGGGACCAATAGATGTGCAGTGTTCAGAGATTAA186350.10369294912429398No Hit
TATTAACCCTCACTAAAGGGATCTGAATGTATGACAAAAGTGCAACATAA186250.10363730493372554No Hit
TAATACGACTCACTATAGCCTATAACTAGCTACAGGGTGATAACATAAAG185830.10340359933333808No Hit
TATTAACCCTCACTAAAGGGAGCTCCCATGTCGTCCTCATTTTCTTGAAA185250.1030808630280411No Hit
TAATACGACTCACTATAGCCGCTCCTTTGATGGATATAAGAATGCAGAAG185090.1029918323231316No Hit
TAATACGACTCACTATAGCCTACAAGAGTGCAGACAAGGGTGATGGGAAG184360.10258562973198197No Hit
TAATACGACTCACTATAGCCGCATGCCTATGTCTGTGCTTAGAAACTAAG183100.10188451293081958No Hit
TATTAACCCTCACTAAAGGGAAGAATGTGAAGAGATCCCAAGACCAGCAA182830.10173427361628477No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGC181500.10099420588172449TruSeq Adapter, Index 4 (98% over 50bp)
TATTAACCCTCACTAAAGGGATCAAGCCAGCTATTGAACATTTCACAGAA181050.10074380702416649No Hit
TAATACGACTCACTATAGCCTGCTGACAAGTAATTGAACAGCAATTAAAG180380.10037099094735794No Hit
TATTAACCCTCACTAAAGGGAACTGTTGACAGCCTAAATATACCAAGAAA180210.10027639582339157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG7235900.069.7440261
TATTAAC7417950.069.7281
AATACGA7410050.068.181032
ATACGAC7415600.068.1697543
ATTAACC7589350.068.1300662
TACGACT7424600.068.10564
ACGACTC7451450.068.0329745
CACTATA7555750.067.9323911
ACCCTCA7635350.067.872756
CGACTCA7486100.067.8713466
CTCACTA15263750.067.827839
ATAGCCC1748650.067.8015115
TAGCCGT343850.067.7598616
ACTATAG7580400.067.7281912
TTAACCC7625400.067.7240453
TATAGCC7558500.067.7038314
TAACCCT7629450.067.699214
TCACTAT7583600.067.6204410
GACTCAC7540150.067.586217
AACCCTC7657600.067.576425
Produced by FastQC (version 0.11.2)