FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_mb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_mb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612712
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC182121210.340326918421194TruSeq Adapter, Index 2 (100% over 50bp)
TATTAACCCTCACTAAAGGGAGCATTTGTTCCCATCTTTTCCAGGAGGAA2576391.4628014129794435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2110841.1984752830796304No Hit
TAATACGACTCACTATAGCCAGTGCGACCAAAGACTTGAGACCAGGGAAG1258360.7144612368611943No Hit
TAATACGACTCACTATAGCCTCATGTCCTTTGGCTGTCTACTCAGAGAAG883960.5018875003463408No Hit
TAATACGACTCACTATAGCCCCCGGTGTCTGTTCAGTGTTGTTAGAGAAG495200.28116056175789395No Hit
TAATACGACTCACTATAGCCATGCCAACTGGAAGCCTAGTACCATAGAAG412290.23408660744580392No Hit
TAATACGACTCACTATAGCCACAAAGCCAAGAGCCATCTGAGAAAAGAAG393660.22350902007595422No Hit
TAATACGACTCACTATAGCCGAGTGAGGAGAAAAGTGGCAATGGAAGAAG392140.22264600704309478No Hit
TAATACGACTCACTATAGCCGGTAAAAACCTCCAAAACGATGAGCCAAAG348300.19775489430588544No Hit
TATTAACCCTCACTAAAGGGATTGGGTGCAGCCGAACAGAGAAGGGCAAA344550.19562575031034402No Hit
TAATACGACTCACTATAGCCGAGACTAGGAGAGACCAAGAATAACAGAAG329770.1872340841092502No Hit
TATTAACCCTCACTAAAGGGAGAGATGCCAGATTAGAAATCAGTCAGAAA326270.1852468830467449No Hit
TAATACGACTCACTATAGCCGTCCAGGTCACTTACTGCTCAGAATAAAAG324440.18420786077692067No Hit
TATTAACCCTCACTAAAGGGACTCGGTGGAGTCTAGCCGGAATGCCAAAA323100.1834470466558472No Hit
TAATACGACTCACTATAGCCTAAACAGTCAAGACGCACAACTCAAAAAAG299290.16992840171348966No Hit
TATTAACCCTCACTAAAGGGAGAATTTGAGTTTGGCAAAAACAAATTGAA292160.16588018926330028No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC279190.15851618989738775TruSeq Adapter, Index 2 (98% over 50bp)
TAATACGACTCACTATAGCCACCAGCGACAACCCAGACAAAAGATGGAAG278120.15790867414399326No Hit
TATTAACCCTCACTAAAGGGAGCTCCACAGTTGGCAGGTGATAACACGAA275210.15625645840345315No Hit
TATTAACCCTCACTAAAGGGAATCAGGAACAGCACTCTCGTAACAACGAA270840.15377529593398223No Hit
TATTAACCCTCACTAAAGGGAGCAGGACAGGCCAGAAAGTTCCCACTTAA269190.15283847257594402No Hit
TAATACGACTCACTATAGCCGCTACCAGGTACAGAGACTCACTGTTCAAG268790.15261136388308627No Hit
TATTAACCCTCACTAAAGGGATAGCACAGCAAACTCACAAATTCACCAAA267480.15186758291397712No Hit
TATTAACCCTCACTAAAGGGAGGACTGAGCTCTTTAGGAACAATGAAAAA266230.15115786824879668No Hit
TAATACGACTCACTATAGCCAGGCAAGTGTCAGAGGAGATAACACACAAG265310.15063551825522384No Hit
TATTAACCCTCACTAAAGGGAGGAGTGGGAATGTAATTCAGTGGTACAAA263950.1498633486995075No Hit
TATTAACCCTCACTAAAGGGACATACTACGATAAACAGACAGATGCATAA262280.1489151699068264No Hit
TATTAACCCTCACTAAAGGGAAGAGGGAGTGTCACGGTGTCTATAAATAA261540.14849501882503957No Hit
TATTAACCCTCACTAAAGGGACCACTAACCACTCTGCCCAACTCCAAGAA261350.1483871421959321No Hit
TAATACGACTCACTATAGCCACACGTCAGCTCATAGTAATGAAGAAAAAG257190.14602521179021152No Hit
TAATACGACTCACTATAGCCGTGGCTTGACAACAGATGTTAAGTCCCAAG255500.14506567756288755No Hit
TATTAACCCTCACTAAAGGGACACACAGAGAGTACCTGGGAACAAGGAAA250990.14250502705091642No Hit
TAATACGACTCACTATAGCCAACCAAGGATCATCTTTTGCTTAAATTAAG250480.14221546346752278No Hit
TATTAACCCTCACTAAAGGGACTAATCAGTGGTGAAATGATTGGATCGAA250170.14203945423055803No Hit
TATTAACCCTCACTAAAGGGATGGAAAAAGGATCAACTGGTCAGATTTAA249410.1416079477141283No Hit
TATTAACCCTCACTAAAGGGAGCAGAACCTTCTAAGACTATTGCATTAAA248470.1410742422859126No Hit
TATTAACCCTCACTAAAGGGAGCTGGACTTGGCTAAGAAACAGTCATAAA248110.1408698444623406No Hit
TATTAACCCTCACTAAAGGGACAGAGAAGGCTCACCATACGAACTGTAAA244000.13853630264322722No Hit
TAATACGACTCACTATAGCCCAAAGCACTGTGATGTTTGTTGGAAGGAAG243460.13822970590786926No Hit
TATTAACCCTCACTAAAGGGAGATCTGGAAAGGACATGTATGGTTTGGAA242100.1374575363521529No Hit
TATTAACCCTCACTAAAGGGATCTGATTGATCGATAACCGGAGACTCTAA241170.13692950864125863No Hit
TATTAACCCTCACTAAAGGGACAGGAGCAGATATGGGAGAGTCATTGAAA240930.136793243425544No Hit
TATTAACCCTCACTAAAGGGATGCACACCCAGTAACATGACGTTTTGAAA240070.13630495973589984No Hit
TAATACGACTCACTATAGCCCAAACAACTACTGCAAACTAAATACAGAAG238110.13519212714089687No Hit
TAATACGACTCACTATAGCCCCAGCGAGTGTACCATAAACTCAAGAAAAG237830.13503315105589644No Hit
TATTAACCCTCACTAAAGGGACTGAGCAGTGTGAAGCAGACTCGAGTGAA236350.13419284889232277No Hit
TATTAACCCTCACTAAAGGGATGACACACCAGAGAGGGAAAGGATGCAAA233870.1327847749966047No Hit
TAATACGACTCACTATAGCCTCAATGACTGCACACCTTTCTCTGTAAAAG232810.13218293696053168No Hit
TATTAACCCTCACTAAAGGGAAATCAGTCCAATTTCAGGTACACAATAAA232670.13210344891803147No Hit
TATTAACCCTCACTAAAGGGAAGAGATAGCAGAGCACACCAGAAGTTCAA230980.1311439146907075No Hit
TATTAACCCTCACTAAAGGGACTACTGTGGTTTAAAACATGATACCCAAA230420.13082596252070663No Hit
TAATACGACTCACTATAGCCCAACAGGGTATTTCAATATATCTAGGTAAG226890.128821728306237No Hit
TAATACGACTCACTATAGCCAAACAAAATCAAGGTAAAGCCTCACTTAAG225490.12802684788123486No Hit
TATTAACCCTCACTAAAGGGACCGTTCTTGAACTGGGCATACTGGTACAA224500.12746475386641196No Hit
TAATACGACTCACTATAGCCTCTACACACAGTGCCTTTTGAGTAGTAAAG223740.12703324734998223No Hit
TATTAACCCTCACTAAAGGGACAATGGAAAAGTCTCTGAGCAGTTTAGAA223010.12661877398551682No Hit
TATTAACCCTCACTAAAGGGAGGATCAATGGGGTAAGTCTGGGAGTGAAA222690.12643708703123063No Hit
TATTAACCCTCACTAAAGGGAACCAACACAGGTCAAAATGTTCCAACAAA221970.1260282913840867No Hit
TATTAACCCTCACTAAAGGGACCAATAGATGTGCAGTGTTCAGAGATTAA221440.12572737236605014No Hit
TAATACGACTCACTATAGCCCCGAGGACCTGTATTCTTGAGACAATAAAG221060.1255116191078353No Hit
TAATACGACTCACTATAGCCGGGCCATATAGAATCCTACTACAGTAGAAG220760.125341287588192No Hit
TATTAACCCTCACTAAAGGGACAGTGAGAAAAACCTCGTCTGGAAAGGAA218730.12418871097193891No Hit
TATTAACCCTCACTAAAGGGAGTCCAGAGTTATTATCGAGAAGACAGGAA218050.12380262619408072No Hit
TAATACGACTCACTATAGCCATGCTGCAGCTAAGACAAGAGTGTTCGAAG215440.12232074197318392No Hit
TATTAACCCTCACTAAAGGGAATGGAGCAAGAACCAAAGATCTGCTTGAA214840.1219800789338973No Hit
TAATACGACTCACTATAGCCGGGATTTCTTTCATTCTGCTCTTGAAGAAG214730.12191762404336141No Hit
TATTAACCCTCACTAAAGGGACTGCATAGCTGGTAATGCTGTTGTTAGAA214300.12167348219853932No Hit
TATTAACCCTCACTAAAGGGAGTGGGTAAGAGCTAACAAAGTTGGGACAA213930.1214634066576459No Hit
TAATACGACTCACTATAGCCTGCTCTCAAGCAACTTCATATACAAGAAAG213470.12120223166085949No Hit
TAATACGACTCACTATAGCCGAGCTGGAGGAGCAGTCACAACACAGCAAG212740.1207877582963941No Hit
TATTAACCCTCACTAAAGGGAGCAGAGGTAGGTAGGAGAGTTGTCTAGAA211660.12017456482567818No Hit
TAATACGACTCACTATAGCCCAAGATGGTAGAGTCAGTGCACCAGGGAAG211550.1201121099351423No Hit
TAATACGACTCACTATAGCCTGAGTGTTCTTGGCACCTTCAATTGGAAAG210480.11950459418174782No Hit
TATTAACCCTCACTAAAGGGAAGAGGAAACCCAACCAGCTTCTCTGCAAA210260.11937968440067605No Hit
TAATACGACTCACTATAGCCGTCAGAGCTCCTCACGTAATGCAACCGAAG209430.11890843386299624No Hit
TAATACGACTCACTATAGCCTTCGCACACTTGTCTCGAGCCTCTGCCAAG208740.11851667136781661No Hit
TAATACGACTCACTATAGCCACCAGAGCAATTTGTTTTTAGGAGTTGAAG208430.11834066213085184No Hit
TAATACGACTCACTATAGCCAGCCTTGCTATGTGCAGAAGGAGACTGAAG208300.11826685180567308No Hit
TATTAACCCTCACTAAAGGGAGAGAGAAACTTAGCGGGGACCTTGATCAA206610.1173073175783491No Hit
TAATACGACTCACTATAGCCACCTAAACCCTGGACTTGTCACTGTATAAG204600.11616609639673892No Hit
TATTAACCCTCACTAAAGGGACAGAGAGTAATTAGCGAAAAGTAAGCCAA204210.11594466542120259No Hit
TATTAACCCTCACTAAAGGGAAGCCTACAGTTGGGTGTTTGAGAAGACAA203590.11559264694727309No Hit
TAATACGACTCACTATAGCCTCTGGGGACAGAACACGGTTCCTTTGAAAG203460.11551883662209432No Hit
TAATACGACTCACTATAGCCTAGAGGGCCAAGGGTACAGGATCTTCAAAG203420.11549612575280854No Hit
TAATACGACTCACTATAGCCCAACACAATGAGAAAGACTATAATAGAAAG202360.1148942877167355No Hit
TAATACGACTCACTATAGCCCACTTGTGGGAGTGTGTGGATTTGTTCAAG201910.11463879043727054No Hit
TAATACGACTCACTATAGCCAACATCGAGACTTAGATACCGTTCCACAAG201240.11425838337673382No Hit
TAATACGACTCACTATAGCCGGACCTTGAGGACACAGTCATAGCCTGAAG201030.1141391513129835No Hit
TAATACGACTCACTATAGCCCCCAAATGTTGTCTACCACTGTCGTTAAAG200970.11410508500905484No Hit
TAATACGACTCACTATAGCCGAACCAGAGAGATGAAGGTACTCGTTCAAG199950.11352595784226757No Hit
TATTAACCCTCACTAAAGGGACAAGCCCAAATCCTGTAGCTGCCTTTAAA199360.11319097252030239No Hit
TATTAACCCTCACTAAAGGGAACTTGACACATCAACAGATCACTATTAAA199040.11300928556601617No Hit
TAATACGACTCACTATAGCCCTGCTAAAACACTATGACAAATCATAAAAG198970.11296954154476607No Hit
TATTAACCCTCACTAAAGGGAAACAAAGCAAACTCATCCGCTCATACCAA198870.11291276437155164No Hit
TATTAACCCTCACTAAAGGGACAATTAATCAACACCAGGATTTGAAGTAA198530.11271972198262255No Hit
TAATACGACTCACTATAGCCGGAAGCAGATAAATCGCACATCTCAAAAAG197720.1122598268795856No Hit
TATTAACCCTCACTAAAGGGACGCTCGAAACTGTGGTTCAGGGATTGAAA195880.11121512689243997No Hit
TAATACGACTCACTATAGCCACCCCAGCCAAGTCTAGCCCAAACAACAAG195770.11115267200190407No Hit
TATTAACCCTCACTAAAGGGATAAGAGGCTAAAGATACAAGGCTTAGGAA194770.1105849002697597No Hit
TATTAACCCTCACTAAAGGGAATGAAAGGCAAAGATAAGGCAGCTTATAA194230.11027830353440174No Hit
TATTAACCCTCACTAAAGGGATCTGCACTCTGTGTTCAACTAGTCCTCAA193270.10973324267154314No Hit
TAATACGACTCACTATAGCCTCCATAGCAATCATATTGCAAATCTGGAAG192680.10939825734957796No Hit
TATTAACCCTCACTAAAGGGAGCCCATTAATTCTGCCATCTCTGAGTAAA192050.10904056115832701No Hit
TAATACGACTCACTATAGCCTCGACTCCATTCCAAAGCAGCGTTTCAAAG191510.10873396442296905No Hit
TAATACGACTCACTATAGCCCGGCAGACGTCTTTGTTCTTTAGCAGAAAG190080.10792205084600259No Hit
TATTAACCCTCACTAAAGGGAACAAGACAGATCAGCAATTAGTCAACAAA189770.10774604160903785No Hit
TAATACGACTCACTATAGCCCAGACTCCAGAAATAGAACATATGCTAAAG189290.10747351117760855No Hit
TAATACGACTCACTATAGCCAGAGTTCCTCATCCAAGGATGTCACCCAAG187700.10657075412349899No Hit
TAATACGACTCACTATAGCCTGTGGAGGAGAGTGGTAGTTCAATTCAAAG187500.10645719977707009No Hit
TATTAACCCTCACTAAAGGGAGTAAGGTATATGTCTGGTGTATCTGGGAA186850.10608814815117626No Hit
TAATACGACTCACTATAGCCTACAAGAGTGCAGACAAGGGTGATGGGAAG186700.1060029823913546No Hit
TAATACGACTCACTATAGCCCACTTAGGAGATAGCTGGAAAGATTGAAAG186680.10599162695671173No Hit
TATTAACCCTCACTAAAGGGACACCAGGTGAATCTTACTTCCACATACAA186530.10590646119689007No Hit
TAATACGACTCACTATAGCCGATAAGGTTACTGATCCCGATAAAACAAAG185600.1053784334859958No Hit
TATTAACCCTCACTAAAGGGAGGTGTCACTTCCATTAAACAATTTCTAAA183660.10427695632563572No Hit
TATTAACCCTCACTAAAGGGAAAGCACTCAAAAGCTCTGGAAACATCAAA182930.10386248296117033No Hit
TATTAACCCTCACTAAAGGGATCAAGCCAGCTATTGAACATTTCACAGAA182180.10343665416206205No Hit
TATTAACCCTCACTAAAGGGAACATATACACTGGAGGTGCCAGAGATAAA180510.10248847536938094No Hit
TAATACGACTCACTATAGCCATCATTTAGGGGAGAATGACCAGGAAAAAG179950.10217052319938008No Hit
TATTAACCCTCACTAAAGGGAGTGTGAGCCCAAAGACTTATGTGTTGAAA179750.10205696885295121No Hit
TAATACGACTCACTATAGCCTTACTGCCCACCTACATCCATATTCCAAAG177700.10089303680205525No Hit
TATTAACCCTCACTAAAGGGACCCGAGGTGGAGAGAGGAGAGTCCTGGAA177670.10087600365009092No Hit
TATTAACCCTCACTAAAGGGAGGTGGATGGGCCTTTCCCATGATTTCAAA176420.10016628898491045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAC7077050.069.763171
TAATACG6777750.069.760391
ATACGAC6906050.068.5492553
TACGACT6911550.068.507754
AATACGA6908950.068.486922
ACGACTC6931950.068.4567265
CACTATA7024600.068.39711
ATTAACC7222200.068.303692
CGACTCA6963650.068.297686
ATAGCCC1649750.068.2501515
TAGCCGT314900.068.2207316
CTCACTA14339800.068.212149
ACTATAG7047550.068.1861912
TATAGCC7025750.068.1483314
TATGCCG2115800.068.1248446
TGCCGTC2118750.068.1158948
ATGCCGT2119950.068.0822847
ACCCTCA7260450.068.080676
TCACTAT7051700.068.0633710
CCGTCTT2108950.068.0525750
Produced by FastQC (version 0.11.2)