FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_mb10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_mb10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15926689
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC4224342.652365472823636TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG767840.4821089932753757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT511200.064.2955942
TCGTATG528600.064.1921943
ATGCCGT529450.064.16164447
TCTCGTA496000.064.09984641
CGTATGC533200.063.99901244
CCGTCTT531200.063.52859550
TATGCCG536500.063.259846
ATCTCGT499350.063.05322640
TGCCGTC539050.062.99950848
GCCGTCT538600.062.850949
CGTCTTC539150.062.76690751
CATCTCG503650.062.45235439
GTATGCC547550.062.15555245
GCTTGAA554850.060.34380359
TAGGCAT550150.059.3636935
TGCTTGA566200.059.17743358
TTAGGCA576100.058.88273234
CTTAGGC590950.058.16099533
ACTTAGG596100.057.97576532
CGTCTGA611150.057.7372816
Produced by FastQC (version 0.11.2)