FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_ba53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_ba53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22763704
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2145980.9427200423973181TruSeq Adapter, Index 10 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG766510.33672463848589845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT254800.063.87236442
TCGTATG260750.063.82452443
CGTATGC261300.063.77041244
TCTCGTA252100.063.64001541
ATGCCGT262250.063.3654947
TATGCCG268900.061.8507746
TGCCGTC268400.061.80910548
GCCGTCT268800.061.67792549
CCGTCTT268950.061.53942550
TATCTCG260250.061.14974639
ATCTCGT262100.061.10524440
CGTCTTC275800.060.29029551
CGTCTGA295250.058.26380516
GTATGCC286100.058.20564745
ACGTCTG298550.057.53797515
ACACGTC301100.057.21330313
GCTTATC281700.056.70559336
GATCGGA308150.055.4512181
ATCGGAA310800.055.3428272
CACGTCT313100.054.97592514
Produced by FastQC (version 0.11.2)