FastQCFastQC Report
Fri 4 Nov 2016
HVLTKBGXY_n01_ba52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTKBGXY_n01_ba52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21464628
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC5050902.353127200713658TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG732610.34131036419545685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT620300.063.70253442
CGTATGC641800.063.68428444
TCGTATG641800.063.60263443
TCTCGTA611600.063.56643741
ATGCCGT643700.063.38712347
CGATCAG654250.063.0558133
GATCTCG600100.063.04672639
TATGCCG647750.062.8828846
ACGATCA657000.062.86142332
CCGTCTT648550.062.842850
TGCCGTC650600.062.77404448
GCCGTCT650450.062.6913749
CACGATC659950.062.64937631
GTCACGA662550.062.52473429
ATCTCGT603950.062.48270840
CGTCTTC655650.062.3759551
GATCGGA685050.062.168011
ATCGGAA688850.062.113442
CGTCTGA685750.061.98724416
TCACGAT668900.061.8478930
Produced by FastQC (version 0.11.2)