FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_H1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_H1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC200.45004500450045004No Hit
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG160.36003600360036003No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA140.31503150315031503No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC130.2925292529252925No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA130.2925292529252925No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT130.2925292529252925No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA100.22502250225022502No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT90.20252025202520252No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA80.18001800180018002No Hit
GAATATAACTGAAGCAAACCTGAGTTAGAAGAATGGAACAAAAATTTTCT80.18001800180018002No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG80.18001800180018002No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA80.18001800180018002No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC80.18001800180018002No Hit
CCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTCTTG80.18001800180018002No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG80.18001800180018002No Hit
AAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAA70.15751575157515751No Hit
AGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAA70.15751575157515751No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT70.15751575157515751No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC70.15751575157515751No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG70.15751575157515751No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC70.15751575157515751No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA70.15751575157515751No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT70.15751575157515751No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC70.15751575157515751No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA70.15751575157515751No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC70.15751575157515751No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA70.15751575157515751No Hit
ACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGAGAAGATTGCTATGG60.135013501350135No Hit
GTATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCT60.135013501350135No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60.135013501350135No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC60.135013501350135No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC60.135013501350135No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA60.135013501350135No Hit
CTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTTG60.135013501350135No Hit
ACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATACC60.135013501350135No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC60.135013501350135No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG60.135013501350135No Hit
GGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGGA60.135013501350135No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC60.135013501350135No Hit
CTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCA60.135013501350135No Hit
ACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATTGGATA60.135013501350135No Hit
CTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATC60.135013501350135No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG60.135013501350135No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC60.135013501350135No Hit
GTGTATAATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCA50.11251125112511251No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA50.11251125112511251No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT50.11251125112511251No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT50.11251125112511251No Hit
CTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTG50.11251125112511251No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA50.11251125112511251No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG50.11251125112511251No Hit
GATTTATTGCATACATAAATAATGAAACTACCTAAGTGCATAGCTAATAG50.11251125112511251No Hit
CTATTTATTTGTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGA50.11251125112511251No Hit
TTTCAAGGCTGATGTGCCAACAAAGTATATTAACTAGAGAATTGAGTGTA50.11251125112511251No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT50.11251125112511251No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT50.11251125112511251No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA50.11251125112511251No Hit
TTATTATCCAACAGTGACTTTTGTTCCTTATTGGTTTAATTTGCATCATA50.11251125112511251No Hit
AGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGC50.11251125112511251No Hit
CTGTTGGATAATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAG50.11251125112511251No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC50.11251125112511251No Hit
CATCTGTATGAATAAATTAAAATCTTATAATTAAATAACTATCCATAAAA50.11251125112511251No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC50.11251125112511251No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA50.11251125112511251No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG50.11251125112511251No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA50.11251125112511251No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA50.11251125112511251No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA50.11251125112511251No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.11251125112511251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)