FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_H10b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_H10b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG220.2796491674081607No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC190.24151519003432056No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC150.19066988686920044No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC150.19066988686920044No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA140.17795856107792044No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT140.17795856107792044No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA140.17795856107792044No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA130.1652472352866404No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA130.1652472352866404No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT130.1652472352866404No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA130.1652472352866404No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG120.15253590949536036No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA120.15253590949536036No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG110.13982458370408035No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG110.13982458370408035No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG110.13982458370408035No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG110.13982458370408035No Hit
GTATGATGATACAAGTATGCCTCATTGGATAAAAGCCACTAGCCCCATTC100.12711325791280031No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG100.12711325791280031No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC100.12711325791280031No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA100.12711325791280031No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT90.11440193212152026No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA90.11440193212152026No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC90.11440193212152026No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG90.11440193212152026No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC90.11440193212152026No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC90.11440193212152026No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC90.11440193212152026No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80.10169060633024025No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC80.10169060633024025No Hit
GTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACT80.10169060633024025No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA80.10169060633024025No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA80.10169060633024025No Hit
CATAGATTCTACAAAGAAACTGTTTCCAAACTGATGAATCAAAACAATGG80.10169060633024025No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG80.10169060633024025No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT80.10169060633024025No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC80.10169060633024025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)