FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628596
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG1464502.6018921947853424No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA976311.7345533415437882No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA721531.2819004952567212No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA694871.234535219795487No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA668891.1883780608876529No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA615321.0932033494676114No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA568301.0096656430839945No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT541780.9625490975014018No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG510280.9065848748071456No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA484500.8607830442973702No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT463490.8234557960812963No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA427730.7599230785083883No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG415170.7376084551102975No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG390870.6944360547461569No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT389800.6925350478165425No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG369600.6566468796126068No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC328540.5836979594911413No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA306590.5447006678041914No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA301960.5364748153891308No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG276540.49131257599586114No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG235320.4180793931559487No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC211000.3748714599520022No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA210870.37464049649326403No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG205110.36440703862917145No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC202600.35994766723353394No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG202380.3595568059956693No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA194170.34497057525535674No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC194000.3446685461170068No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA189580.336815788519908No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC188100.3341863583742731No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG186380.33113053415096766No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT186290.3309706363718412No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT175940.3125823917722999No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC175530.3118539685562794No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG171760.3051560282528716No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT164410.2920977096242118No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT161590.2870875792115831No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA159110.28268150707565437No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC150830.2679709113960213No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT145790.25901663576494033No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT141920.25214103126250315No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG139130.24718420010958328No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA137810.2448390326823954No Hit
ATATTACGTAGTGTGTATACACACACACACACACACACACACACACACAC137520.24432380650521016No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC136680.24283142723336335No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA136440.24240503315569284No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC133010.23631115112898493No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC132040.2345878083983999No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA129830.23066142959985045No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG128930.229062451808586No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG127760.2269837806799422No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA125160.22236451150517822No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT124350.2209254314930402No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT123970.2202503075367285No Hit
ATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAA123800.21994827839837855No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG123220.21891782604400814No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA119480.21227318500030914No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA119190.21175795882312393No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA115640.20545087975758075No Hit
TGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATT115170.20461585802214266No Hit
GTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTAT114330.2031234787502958No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA113870.20230622343476062No Hit
TCTTAATATTACGTAGTGTGTATACACACACACACACACACACACACACA112800.20040521650514623No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT111660.19837984463621122No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT108820.1933341813837767No Hit
GGTTTAACTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGC108230.19228596260950334No Hit
GTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGTCC108220.1922681961896004No Hit
GTATGTAAGTGATCTAAATACAGCTGCCTGAAGGTATCAAGTAAATCCGT107090.19026059074056834No Hit
AGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCA101580.1804712933740492No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT99990.177646432609482No Hit
ATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACC99180.176207352597344No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT97890.17391548442986493No Hit
TCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCC95810.17022006909005372No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC95260.1692429159953921No Hit
GAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCAC94340.16760840536432176No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA94000.16700434708762185No Hit
CAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAG93710.16648912091043663No Hit
ATCATAAGCATTACTATTTGAAAAAAATCCCAGCAGCTGCCTAAAACCTA90860.16142569123809916No Hit
AAAGAGAGGTCCTCCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGG90460.16071503444198162No Hit
CTATAAAACAAAGAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTC90110.16009320974537877No Hit
CAGTAAATACAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTT89630.15924042159003773No Hit
GCATAAAGTACATTAAAAATTTTTTTCTGCGACTTGTCTTGATCTGAAGT88350.1569663198424616No Hit
TGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGG87900.15616683094682937No Hit
ATTAGAAAGAGGTCACTTCTCTGCCATTTCTATTTCTCTGAAGATCCTTA86900.15439018895653553No Hit
GAATAGCGATCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATG85730.15231151782789173No Hit
GATTACTACCATTCATCAACTATTCCTCTATCTTCAAGACCTTACTGATG85160.15129883189342422No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT84520.15016178101963618No Hit
GAATCACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCT83870.14900696372594516No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACACTA83660.14863386890798344No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA83570.148473971128857No Hit
GTCTTGATCTGAAGTAAATGTACTTCTCTAATGTATCAGCCACTGAGTCA83170.14776331433273945No Hit
AACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTAC82630.14680392765798078No Hit
GAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTT81360.1445475923303076No Hit
TGATTACAGAGAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATC80820.14358820565554892No Hit
TCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAA78790.1399816224152524No Hit
CTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTT78180.13889787080117316No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG78180.13889787080117316No Hit
GAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAAC77900.13840041104389086No Hit
GGATGAATATTTGGAGGCAAAGAGAGGTCCTCCCTTATTTCTCTGTAATC77410.13752985646864688No Hit
CTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAA77270.13728112659000574No Hit
GTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCACAGTTTAT76170.13532682040068253No Hit
ACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGA75770.13461616360456496No Hit
CCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACT75130.1334791127307769No Hit
ATTTTAATGATATGAGACCTCATTTGTTTCTAATAGGGAGACAGACAGTT74020.13150704012155073No Hit
ATAGATTACTTGGAAGCTCAAAGAATTGGCTGATTATGATTTATCTCCTC73460.1305121206069862No Hit
TCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAA73150.12996136158999508No Hit
CTGCAAATGAGCACAAAGATCATTCTGGGATGATGAAAATGTTCTAAAAA73110.12989029591038334No Hit
GATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTT72890.12949943467251868No Hit
GTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGTCCTCCC72520.12884207713610998No Hit
AGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACT70290.12488016549775467No Hit
ATATAGTACACTGCTACTTGCAAATTTTATAGCAGCTCTGTAAAAGATTT69220.12297915856814025No Hit
ATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATA69160.12287256004872263No Hit
GTCTTATTGTGATTTTAATTTGCATTTCCTTTATGACTAATGATGTTTCT68940.12248169881085799No Hit
AATCATAGGTGATTCAGGTGGTTTAGTTATAAGTAAGGACTATTTGATCA68870.12235733387153741No Hit
GTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAA68350.12143348003658462No Hit
AAAGAATAGCGATCTCAGAAGAGTATCAAGAACAATTCAGAACATACAAT68320.12138018077687579No Hit
ATTTAAGGAAATGCTCTGGTCTTCAGCCCATCAGTAAGGTCTTGAAGATA68000.12081165533998177No Hit
GAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGG67570.1200476992841554No Hit
GAATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCC66820.11871521779143503No Hit
ATATGAGACCTCATTTGTTTCTAATAGGGAGACAGACAGTTTAAGCAGGA66730.11855532001230859No Hit
ACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGT65610.11656548098317947No Hit
GTTCTACAGGTCCCTGTGTGATCACTTACTGGCTCAACAGATCTGTTGAG65310.11603248838609131No Hit
GTTCTTATACTATAGGAACAAAAACTACTGCCACAGAATGTAATGCTACT64970.11542843010939138No Hit
CATCTAGAGATTTCTTAATATTACGTAGTGTGTATACACACACACACACA64920.11533959800987671No Hit
AGTTAGCATTGCTTTAAAAAATAATCTCTGTTATAGCAGGTTCGTACTCT64660.11487767109240031No Hit
ATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAGGT64550.11468224047346798No Hit
CTATGAAGAAGGTCTATTATTACCCCAATTTATAGAAAGGCCCAAGCCTT64090.11386498515793281No Hit
TTTCTAGGCAATCTAGTTAGTTGTCATCCGTGCAACTAATGGAACCAATA64000.11370508737880637No Hit
ATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAGG63920.11356295601958286No Hit
ATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTT63620.1130299634224947No Hit
AAATACAGCTGCCTGAAGGTATCAAGTAAATCCGTGGCACAGAAACCTCA63160.11221270810695953No Hit
TCCCAGGACCCTGAGATCAGGACCTGAGCCAAAGGCAGATACCTAACCGA62660.11132438711181261No Hit
ACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTC62600.11121778859239499No Hit
TTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATAC61630.10949444586180995No Hit
GTTTGAGTATGCAGCTATCATCACAGTTTATTTTTAGAACATTTTCATCA61010.10839292782782775No Hit
GTCTAACTCCTTTTCATGAAACCATTTGAAAAAAGTATGTGGAAAGACAG60010.10661628583753392No Hit
GTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCACAG59070.10494624236665767No Hit
GTATTTACCAGTACACAAGAATAAAGGATTAAAACAAAGAATGGGGGCAC58530.10398685569189901No Hit
CAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACC58120.10325843247587854No Hit
GGAATAATGTTCCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGG57940.10293863691762564No Hit
GTAAATGTACTTCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGG57890.10284980481811093No Hit
AATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGATGATGATTA57670.10245894358024629No Hit
ATAAAGAGTTGAGGCATGATAATTTAGTAAATGGGACAAGAATGATACCT57490.1021391480219934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)