FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208547
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAAT172600.4101177912471929No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT152660.36273801860832255No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC145790.3464140949358532No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG139320.33104061805654067No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA105160.24987246192094328No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT103560.24607067474831573No Hit
GTCCTAATCTCACCTGGGGGCTATTTCTAGTAGACTCCTGGTGGAGCCTT99610.23668501266589156No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT99150.23559199885376117No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG90540.2151336316310594No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA88610.21054772585407744No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG86030.20441734403821554No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT85620.20344313607522976No Hit
GTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAATTA85240.20254021162173075No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG84820.201542242488916No Hit
GGTTCACACAGTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAG84780.20144719780960033No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG83580.1985958574301297No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT83070.1973840377688547No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG82260.19545938301271198No Hit
ACCTACGCAAGTGCAGAGCTTGGGCTGGAAGTTTCCAAGGGGCTCAAGGA79150.18806965919591728No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC75770.18003838379374162No Hit
GTCTGAGGAGGAGGCCGAGCTGCGGACGGGTGAGGGATGACATCGGGCAG73170.17386047963822193No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC70340.16713606857663701No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT68850.16359565427212763No Hit
GCTCAGGAGAGGGCGCAGGCTGTCTGAGGAGGAGGCCGAGCTGCGGACGG68740.16333428140400952No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA67460.16029285166590748No Hit
GCCCTGGAGCCTCTGCGTTCTCAGCTGCCACTCGGGGCTCTTCGGTGCCT67410.16017404581676287No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC66960.15910479317446138No Hit
GTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTAACTAGTGGGAAACT66470.15794049585284423No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA62100.14755686463760534No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA61680.14655889550479062No Hit
GCTCTGGCTGGGTTGGAGAGTGGCAATGGAAAAGAACCCCAGGCAAGCCC60800.14446791255984548No Hit
ATCTTATCTTACCTGTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTA58900.13995329029235032No Hit
GATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTT58400.13876523180090422No Hit
GTGCAGAAGGGGCCCTTTGCCAAGGAGCCTGGCTCTGGTTCAAACAGAGG58390.13874147063107528No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT58020.13786230734740518No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT57580.1368168158749326No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG56920.13524857866622375No Hit
CTTCTGCACATCGGCTTCTCTGTCTGCGAACTGAGAAGACAAAAATGACG55920.13287246168333156No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG55660.1322546712677796No Hit
CCCCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCA55410.13166064202205655No Hit
GTTTATACCTTGGTTCTATCTTATCTTACCTGTCCAGGTAGTGAGCTGGC54240.12888058515207268No Hit
GATAAAGCCAGTAGCCCAAGGTCACACAATTAGGCTTTGACATGGTGGGA53870.12800142186840258No Hit
CTTCTGGAAGGAGGTGGAGTCTACAGAATGACTTGCTCACTTGATTGGAG53640.12745491496233735No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT53290.12662327401832507No Hit
ATCTTGAGCAGCTCTGGCTGGGTTGGAGAGTGGCAATGGAAAAGAACCCC52560.12488870862081378No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG51960.12346303843107846No Hit
CCCTTGGGGTGACAGGAAAACAGTGCAAAGGAATCCTAGAGGCTCAAGCC51750.12296405386467112No Hit
CCCCAAGGGAGGTTAGATGCTGGGAGCCAGGAATCTCCTCTGTTTGAACC51540.12246506929826376No Hit
GTATAAATTCAGTCTCCCACCCAGAGGAGTTTGGTGGCTTGAGCCTCTAG51540.12246506929826376No Hit
GTTCAGAAGTGAGGCAGCTGCTCCTGCCCTTCTTGGGATAGATCTTGAGC50830.12077802624041029No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA50800.12070674273092351No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50660.12037408635331862No Hit
TTAGAGCACAGTGTCCTAATCTCACCTGGGGGCTATTTCTAGTAGACTCC50640.12032656401366078No Hit
TCTTGGGATAGATCTTGAGCAGCTCTGGCTGGGTTGGAGAGTGGCAATGG50540.12008895231537156No Hit
CTACCTGGACAGGTAAGATAAGATAGAACCAAGGTATAAACAAAAGGCTC50520.1200414299757137No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA50100.11904346084289899No Hit
TATCTTACCTGTCCAGGTAGTGAGCTGGCTTTCTCTCTTGGGCGTAACTA50070.11897217733341221No Hit
GGTGAGGGATGACATCGGGCAGATTCCAGGCGATCAAGTGTCAGTGGCAC49910.11859199861614945No Hit
GTTTTCCTGTCACCCCAAGGGAGGTTAGATGCTGGGAGCCAGGAATCTCC49790.1183068645782024No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT49150.11678614970915141No Hit
TTCCAGAAGAGTATAAATTCAGTCTCCCACCCAGAGGAGTTTGGTGGCTT48810.11597826993496807No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA48710.11574065823667884No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT48450.11512286782112686No Hit
GCCATAAGCACCACATTCCCCCAAAGGGGAATTCTTGAGGATGAACAGAT48260.11467140559437736No Hit
CTCCAGGGCTGATAAAAGTTGTAATTAACGTGCACATACAACCCTTTCAT47980.11400609283916754No Hit
AAGCACCACATTCCCCCAAAGGGGAATTCTTGAGGATGAACAGATGTACC47850.11369719763139154No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA47800.11357839178224693No Hit
CATACAACCCTTTCATCTTCAAAGGCTTTACTCCCATTAGCTAGTTATTC47510.1128893178572082No Hit
CAATTAGGCTTTGACATGGTGGGAATTAGAGCACAGTGTCCTAATCTCAC46920.1114874088373018No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT46400.11025182800619786No Hit
GATTAGGACACTGTGCTCTAATTCCCACCATGTCAAAGCCTAATTGTGTG45650.10846974026902871No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT45580.10830341208022626No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA45430.10794699453279244No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG45130.10723415943792478No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT44990.10690150306031988No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA44960.1068302195508331No Hit
CTTCTGAACTCTGTCCAGCAGCCTCCGAAGAGGGTGTCGTGGGAACTTCC44940.10678269721117527No Hit
TCCTGGGACACTCAAGTTTCCCACTAGTTACGCCCAAGAGAGAAAGCCAG44630.10604610094647868No Hit
GCTCTGTGAGCCGCAACCCTGCGGGCTTCCTGCAAGGGGCGGGATTTGAG44330.10533326585161103No Hit
GCACCACATTCCCCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAG43850.10419272969982277No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA43020.10222055260402224No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT42790.10167404569795704No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAGAGTACACATCAG42510.10100873294274723No Hit
GGTTGTATGTGCACGTTAATTACAACTTTTATCAGCCCTGGAGCCTCTGC42120.10008204731941928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)