FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAAT150.3597122302158274No Hit
GTCTCTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCT140.3357314148681055No Hit
GACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACA140.3357314148681055No Hit
GAATTAGGGAAGATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACT110.2637889688249401No Hit
GTCCCTGGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTC100.2398081534772182No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG90.2158273381294964No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA80.19184652278177458No Hit
GTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCA80.19184652278177458No Hit
GGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAA80.19184652278177458No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC80.19184652278177458No Hit
TTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAAGAGATACCTGGAATT80.19184652278177458No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.19184652278177458No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT70.16786570743405274No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC70.16786570743405274No Hit
GCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAAGAGAT70.16786570743405274No Hit
CTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACAAA70.16786570743405274No Hit
ATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTT70.16786570743405274No Hit
ATTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTTTG70.16786570743405274No Hit
AGCGAAAGCAGGTGGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGA60.14388489208633093No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60.14388489208633093No Hit
GCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTG60.14388489208633093No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG60.14388489208633093No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTT60.14388489208633093No Hit
GCTCCCAGCATGCAATCAGTGCTCAGTCTCTATCTGCACAGACTACAGAA60.14388489208633093No Hit
GCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC60.14388489208633093No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT60.14388489208633093No Hit
ACCAAGCACGGTGCTTGGCACAAACTGGACCTTCAGTAAATAAATTCCAG60.14388489208633093No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT60.14388489208633093No Hit
GTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGG60.14388489208633093No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA60.14388489208633093No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC60.14388489208633093No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC50.1199040767386091No Hit
TCTGTAGTCTGTGCAGATAGAGACTGAGCACTGATTGCATGCTGGGAGCA50.1199040767386091No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA50.1199040767386091No Hit
GGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTCTCTATC50.1199040767386091No Hit
GTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGGAAGCATTGG50.1199040767386091No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA50.1199040767386091No Hit
CTTCTTACCTTTTCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAA50.1199040767386091No Hit
GTCCAGTTTGTGCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCG50.1199040767386091No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC50.1199040767386091No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA50.1199040767386091No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA50.1199040767386091No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.1199040767386091No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA50.1199040767386091No Hit
TTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC50.1199040767386091No Hit
CACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAAATACT50.1199040767386091No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC50.1199040767386091No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC50.1199040767386091No Hit
GTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGGAAGCATTGGTG50.1199040767386091No Hit
GATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAAA50.1199040767386091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)