FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_G1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5555
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCT180.32403240324032406No Hit
GAATTAGGGAAGATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACT150.27002700270027No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT150.27002700270027No Hit
GACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACA130.234023402340234No Hit
GGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAA130.234023402340234No Hit
ATATTTTAGGGGAAAGAGATACCTGGAATTTATTTACTGAAGGTCCAGTT130.234023402340234No Hit
GTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATTGAGGG120.21602160216021601No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC120.21602160216021601No Hit
GGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAA110.19801980198019803No Hit
CTATCTGCACAGACTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAAT100.18001800180018002No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA100.18001800180018002No Hit
GCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTCTCTATCTGCACA100.18001800180018002No Hit
CTACAGAATGTTTGCTGTCACCAATGCTTCCCTCAATGGACGACTACAAA100.18001800180018002No Hit
CACCAATGCTTCCCTCAATGGACGACTACAAAAATCGGACGAGGGACTAC90.16201620162016203No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT90.16201620162016203No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80.14401440144014402No Hit
GCCAAGCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTG80.14401440144014402No Hit
GCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTAGGGGAAAGAGAT80.14401440144014402No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT80.14401440144014402No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA80.14401440144014402No Hit
GTCCCTGGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTC80.14401440144014402No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA80.14401440144014402No Hit
GCAGGTGGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGATATTTTA70.126012601260126No Hit
GCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTCGTCCATT70.126012601260126No Hit
AGCAGGGATTGTCCCTGGTTAAGCTCACCACTGCTCCCAGCATGCAATCA70.126012601260126No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA70.126012601260126No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG60.10801080108010801No Hit
GAGATACCTGGAATTTATTTACTGAAGGTCCAGTTTGTGCCAAGCACCGT60.10801080108010801No Hit
AGCGAAAGCAGGTGGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGA60.10801080108010801No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT60.10801080108010801No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG60.10801080108010801No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT60.10801080108010801No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG60.10801080108010801No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT60.10801080108010801No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA60.10801080108010801No Hit
GCGAAAGCAGGTGGGTATGCCAAGTGCTTTTTAAAGGAATTAGGGAAGAT60.10801080108010801No Hit
ATAAAGATCTGGGTCCTTCTGACCTGGCTATTCAGCGGCTTAACCCCACC60.10801080108010801No Hit
AAAGCAGGCAGGTGCCTAGAGGGCAGGAGAGGGAGCAGGACCCAGGGGCC60.10801080108010801No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT60.10801080108010801No Hit
GCACCGTGCTTGGTACTTGACGTGTAGTCCCTCGTCCGATTTTTGTAGTC60.10801080108010801No Hit
TGGTTAAGCTCACCACTGCTCCCAGCATGCAATCAGTGCTCAGTCTCTAT60.10801080108010801No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG60.10801080108010801No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC60.10801080108010801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)