FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAATGCGGCACAGATTGAAAGCGACAGAGAATAGTTTCGAACAAAT21.2345679012345678No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA21.2345679012345678No Hit
CAGAATTTACTTTGTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTC21.2345679012345678No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG21.2345679012345678No Hit
TCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA10.6172839506172839No Hit
CATTTCTTTCTTTCTTTCTTTCTTTTTTTTTTTTTAAATTTTATTTATTT10.6172839506172839No Hit
GGTAATCCTGGAGCAAGGGTGTGTGTGCATGAACTCAGAGAACAAGCCAG10.6172839506172839No Hit
CAATTGCTCTCTTAGCTCCTCATAATCGATGAAATCACCTGGGTAACACG10.6172839506172839No Hit
CAGCAGCTTCCAGTAAAAATGCATTTCCTTTGGGTGCTCCAGCTCTCGTA10.6172839506172839No Hit
AACCAAAAGGAGGCAGTGCCAGTCAGGATCGAAAGATACCTCCCCCCATT10.6172839506172839No Hit
TGTCTGAACTAGGTGTTTCCACAATGTAGGACCATGAGCTTGCTGTGGAG10.6172839506172839No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10.6172839506172839No Hit
CGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTC10.6172839506172839No Hit
ATGCAGGGCTTGATCCCAGGACCCTGGGATCATGACCTGAGCCAAAGGCA10.6172839506172839No Hit
GAGTCTGCGCTTCCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAAC10.6172839506172839No Hit
ATAATCTACACTGTAGATACCCATTATAGAACATCCAGAAACTGATGGCC10.6172839506172839No Hit
TGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAG10.6172839506172839No Hit
GTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCACGGAGAT10.6172839506172839No Hit
GAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTATCATTT10.6172839506172839No Hit
GATCAGTTGCACATAAATCCTGCCTGCCTGCTTGTGTGTATGGGCTTGCA10.6172839506172839No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT10.6172839506172839No Hit
AAACAACAGCACTGTGACCATCTGTGGGGAAACACCACCCGCTGTCCTCG10.6172839506172839No Hit
GTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAA10.6172839506172839No Hit
GGATGGCATGCCACTCTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTC10.6172839506172839No Hit
GTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAA10.6172839506172839No Hit
AGATACAGAATAAGTTGTTTAGCGCGTCGTTACACTAGAAAAGCCTAGAG10.6172839506172839No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA10.6172839506172839No Hit
GATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCA10.6172839506172839No Hit
GGTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCA10.6172839506172839No Hit
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT10.6172839506172839No Hit
CCTCTAATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAA10.6172839506172839No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG10.6172839506172839No Hit
GATCCCACTGTGTCCAACAGGATCAACAAAACTGGTCCCCAAAGGGGCTA10.6172839506172839No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCTGTC10.6172839506172839No Hit
GCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCT10.6172839506172839No Hit
ATCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAGAT10.6172839506172839No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA10.6172839506172839No Hit
CCCCTAACTGCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCAC10.6172839506172839No Hit
GGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGC10.6172839506172839No Hit
GCTTGGGACATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTG10.6172839506172839No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.6172839506172839No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGGGGCTGTCTC10.6172839506172839No Hit
AACCAACAGAGACACCCCGGCGCCCCTCATTACTTTATATCTTTCTAAAT10.6172839506172839No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT10.6172839506172839No Hit
TAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGAGGT10.6172839506172839No Hit
CTGCTGCAATAGAAGATTTAAGAGTATAAAGTTTCATAAGAGGAAAAAAA10.6172839506172839No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA10.6172839506172839No Hit
CGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAATAACGGCG10.6172839506172839No Hit
GAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAGGATCATGAC10.6172839506172839No Hit
ATCCTGTGGCCAGTCTCAAGTTTGTGCTTTTTACATATTTTGGACATTTT10.6172839506172839No Hit
CATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAA10.6172839506172839No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG10.6172839506172839No Hit
ATCCAACTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCC10.6172839506172839No Hit
GCTTTCCTCTTGGAATCACTTTCTTTCCTCTTATGAAACTTGATACTCTT10.6172839506172839No Hit
GTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAA10.6172839506172839No Hit
GGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTCAACCAGAAT10.6172839506172839No Hit
CCAAGGGGGAGCATGATATGAATTGTTCCCTTATGACAAACACATCCCCC10.6172839506172839No Hit
TCCATTGTCTGAACTAGGTGTTTCCACAATGTAGGACCATGAGCTTGCTG10.6172839506172839No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG10.6172839506172839No Hit
GAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGAT10.6172839506172839No Hit
ATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTCC10.6172839506172839No Hit
CAAAAACAATCTCAAAAAAAAAAAAAAAAAAAATATAAAAAAAAAAAAAA10.6172839506172839No Hit
ATTTTCACCCCTCCAGAAATTTCGGTCATTGATTCCACGTTTGATCATTC10.6172839506172839No Hit
CCTCTGACCTCTCCATTCTCACACTCTCTCTCGCAAATAAATAAAGAAAA10.6172839506172839No Hit
GTCCACCCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAG10.6172839506172839No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT10.6172839506172839No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC10.6172839506172839No Hit
AGTCAGAGGTGACAAGATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCT10.6172839506172839No Hit
CAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCT10.6172839506172839No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT10.6172839506172839No Hit
GAGCTAGGATGAGTCCGAATAGTTCTCATTGCATGTACCATCTGCCTAGT10.6172839506172839No Hit
GATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTT10.6172839506172839No Hit
GCATAAAATTCAGAAAATAAAAAAAAAAAAAATGAAACCAATAATACTAA10.6172839506172839No Hit
GTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATT10.6172839506172839No Hit
GGTGATGGGATTTCCCCTTTGAGAGCAGGGACTGAAGGAAGCAGACTGGC10.6172839506172839No Hit
GCGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTCTGTC10.6172839506172839No Hit
TCTTCATTCCTCACTGGGCCTGGAGATCTGGAGCCACCCAGGCGGGGTCC10.6172839506172839No Hit
GTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGC10.6172839506172839No Hit
CCTCTTGGAATCACTTTCTTTCCTCTTATGAAACTTGATACTCTTAAATC10.6172839506172839No Hit
GAGTTGGCACCACAGCAGCTTCCAGTAAAAATGCATTTCCTTTGGGTGCT10.6172839506172839No Hit
TCTCTGATATGTGGCATCATTCAGGTTGGAATGCCAAATCATGATATGAG10.6172839506172839No Hit
TCACCAAAACAAAAGAATAACACTACCAACGCAATATATTATATCTCCCT10.6172839506172839No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT10.6172839506172839No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT10.6172839506172839No Hit
CACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGC10.6172839506172839No Hit
CTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACCTCTCCATTCTCACACTC10.6172839506172839No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10.6172839506172839No Hit
ACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGC10.6172839506172839No Hit
TCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCTACGATAAA10.6172839506172839No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG10.6172839506172839No Hit
TGTTTATCCACATCAATATGCGCACCCGTAACGTCTTGTATAGCAGCTGT10.6172839506172839No Hit
TTCTAGAAGGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTG10.6172839506172839No Hit
ACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGAC10.6172839506172839No Hit
CCATTGCCGTATTTGAATGAAAACCCTTTTACTCCATTTGCTCCATTAGA10.6172839506172839No Hit
GCACTGCAGAGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATTGGGA10.6172839506172839No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG10.6172839506172839No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA10.6172839506172839No Hit
GCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCC10.6172839506172839No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA10.6172839506172839No Hit
CAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGAAGTAAAATCTGG10.6172839506172839No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG10.6172839506172839No Hit
CTGCTAATGGTTACCTGAGCTGGTGGGTTCCTCGGGCTTCTTGTCCTGGG10.6172839506172839No Hit
CTTTCCCTTTATCATTAATGTAGGATTTGCTGAGCTTTGGGTATGAATTT10.6172839506172839No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA10.6172839506172839No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG10.6172839506172839No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA10.6172839506172839No Hit
AAATAAAGAGAGAGACAAAAATAAACCAGAGGTCAGATGAAGCACATACT10.6172839506172839No Hit
GTAAGGTGGTCAGCCTGATGAGACCAAATGAAAACCCAGCTCACAAGAGT10.6172839506172839No Hit
CCTGTCAGGCTCTCTGCTCTGCAGGGGACCTGCTTCCTCCAATCTCTGCC10.6172839506172839No Hit
AAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAA10.6172839506172839No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG10.6172839506172839No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA10.6172839506172839No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA10.6172839506172839No Hit
ATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAA10.6172839506172839No Hit
CTCCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCT10.6172839506172839No Hit
TTAATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAG10.6172839506172839No Hit
ATATAAAATAGATATTGAAATAATCGAAAGATAACATAAACATAATAATA10.6172839506172839No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC10.6172839506172839No Hit
AAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGG10.6172839506172839No Hit
CTTGTCATATTGCATTGGCCAGAATCTTCAGTATCAAGTTGCATACTGGC10.6172839506172839No Hit
GTCTAATGGAGCAAATGTAGTAAAAGGGTAATCATACAAATACGTCAAAG10.6172839506172839No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAG10.6172839506172839No Hit
ATGGGAACAGTGACCACAGAAGCTGCTATTGGTCTAGTGAGTGCCACTAG10.6172839506172839No Hit
GTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAAT10.6172839506172839No Hit
TGTCATGATGGCATCAATTGGCTAACAATTGGAATTTCTGGCCCAGACAA10.6172839506172839No Hit
GACAGGAATTAGTTGACCAGTATAGTCTAATGGAGCAAATGTAGTAAAAG10.6172839506172839No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA10.6172839506172839No Hit
CTTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAG10.6172839506172839No Hit
GCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTTCTCCTCCTGG10.6172839506172839No Hit
GCTGAATGCTGCCATAACGGTTGCTCTTTCAAAAGGGAGATTCCGCTGCA10.6172839506172839No Hit
TGGCATGCCACTCTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATA10.6172839506172839No Hit
GTACCACCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTT10.6172839506172839No Hit
GCAAGGAGGATCTGTCGCACAAGCCCCTGTGGGGACCAGTTTTGTTGCTC10.6172839506172839No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10.6172839506172839No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT10.6172839506172839No Hit
GAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGGTATTATGGTG10.6172839506172839No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC10.6172839506172839No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA10.6172839506172839No Hit
CTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGC10.6172839506172839No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTCTGTCTCTTATAC10.6172839506172839No Hit
TATCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAATGTG10.6172839506172839No Hit
ATGCATATGTTTTTGTGGGGTCATCAAGATACAGCAAGAAGTTCAAGCCG10.6172839506172839No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAA10.6172839506172839No Hit
GGTCTGGTTAGCTGAGGTGATGGGATTTCCCCTTTGAGAGCAGGGACTGA10.6172839506172839No Hit
AAGCAGGAGAGGTCAGTTACCAAGGCTCATAGAGAGAAGGAAGTTTCTCA10.6172839506172839No Hit
CAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAA10.6172839506172839No Hit
CAAAAAAGAAAGCACAAAAGATAGAAGACTAGAAAGAAAATTTTGTTGAG10.6172839506172839No Hit
ATCTTGATGACCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT10.6172839506172839No Hit
CAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATACATATTC10.6172839506172839No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG10.6172839506172839No Hit
CGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCT10.6172839506172839No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATG10.6172839506172839No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGC10.6172839506172839No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA10.6172839506172839No Hit
AGTTATAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGC10.6172839506172839No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCCTGTCTC10.6172839506172839No Hit
GCATTTCTTTCCATTGCGAATGCATATCTCGGTACCACTAGATTTCCAGT10.6172839506172839No Hit
GATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCA10.6172839506172839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)