FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19440788
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC1466670.7544292957672292No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT1121590.5769262027856072No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG892140.45890115153768457No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT815560.4195097441523461No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC756740.3892537689315886No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC712390.3664409076422211No Hit
GTCTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCA698370.3592292658095958No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG686940.3533498745009719No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA643430.3309690944626319No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA642270.3303724108302606No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA625870.3219365387863908No Hit
GAGTAGCAGTGACGGGTATCTCCTATCATTAGAGGAATTCACATCCATAG623850.32089748625415804No Hit
TCCTTGGACAAGCGCCTCAAACTCATCCACCTCTGCCCCACCAACTTTGG617310.3175334250854441No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACG572080.2942679072473811No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG571560.29400042837769746No Hit
CTCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCT556730.2863721367672956No Hit
CTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAA472100.24283995072627718No Hit
ATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAA463900.23862201470434222No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA439450.2260453640047924No Hit
GGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGGGTCTCCT432010.222218358638549No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC427990.22015054122291752No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA427660.2199807950171567No Hit
ATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGCTGGAAC425030.21862796919548733No Hit
ACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAG423510.21784610788410427No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA422000.2170693903971382No Hit
CCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGA411690.21176610742321764No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA411060.21144204648494702No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA401400.206473112098131No Hit
GCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTA393320.20231690196920002No Hit
GACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTT384190.19762059027648468No Hit
CCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTAT375730.19326891481970793No Hit
CCCCTGTGGGGACCAGTTTTGTTGCTCCTGTTGGACACAGTGGGATCTGG365280.1878936183039494No Hit
ACCTATGGCAAGCAGTTTTCTGGATTTTTCTAAAGGTCTGCCAATTTCCA356730.18349564842741972No Hit
AACTTGGACAGCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAG348640.17933429447407173No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC348240.17912854149739196No Hit
GTGCTAGGAGTTGTCACCACATTGGCCCTTTTTTTCCCAAACACATTTGT346010.17798146865240236No Hit
GTCGTAGATGATCCTCGTGCCTCTGATTAGGAGAGGCCACCATTGGCTGT345400.17766769536296576No Hit
CATTGGAACAGAGAGGCTAGCCCGGATTCGGCAAGGAGGATCTGTCGCAC339970.17487459870453811No Hit
CCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGG338380.1740567306222361No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT337830.17377382027930144No Hit
GTGCAACATCACTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGG336770.1732285748911001No Hit
TCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCTC329630.1695558842573665No Hit
ATCCTGGAAACCACGGAAGCTGGAACGGACAACTTCTTTGACCTTCGTAC327920.16867629028206058No Hit
GTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGG323860.1665878975687611No Hit
GTACAGAGTTACCCAGGAACGCTGAATTTGCTGGAACGTTCTTGTTAAGT321000.16511676378550086No Hit
GCTTTAAGTCAGTGCCATTGCCATTAAGTTCTCCAAACATAAAGCTGAAT320500.1648595725646512No Hit
GTTCCAAGCAGGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTG320110.16465896341238842No Hit
GTACCACCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTT318070.1636096232313217No Hit
GAGCAAAAGCAGGGGGGCGTCCGAGCAATGGGTGAGTTCTTGCACTCCAG317930.16353760968948378No Hit
ATCCCACTGTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTG312050.16051304093229143No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA309770.15934024896521684No Hit
GATCCCACTGTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTT308590.15873327768401158No Hit
CTCCAAGGCACCTGGCTTCAGACCACCTTCCCAGCGAGTTTCTACTAGTC308060.1584606549899109No Hit
GTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTAC305410.15709754151940752No Hit
ATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTC303880.1563105363836075No Hit
CTTTAAGTCAGTGCCATTGCCATTAAGTTCTCCAAACATAAAGCTGAATC299810.15421699984589102No Hit
ATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCA297980.15327567997758115No Hit
ACTCTGTACTTGGACACTTGGAAAATGTGCACCCCGACAACTCCAAGGCA295370.1519331418047458No Hit
TTTTCCAAGTGTCCAAGTACAGAGTTACCCAGGAACGCTGAATTTGCTGG295160.1518251214919889No Hit
AATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGAC294210.1513364581723745No Hit
ACTTTGGGCCAACCAAAAGGAGGCAGTGCCAGTCAGGATCGAAAGATACC294070.15126444463053657No Hit
GGTTAGCTGAGGTGATGGGATTTCCCCTTTGAGAGCAGGGACTGAAGGAA292110.1502562550448058No Hit
TCTCTTTTCTGTCCCCTTTTGGGGCTAGTTAGATTCAGCTTTATGTTTGG290300.14932522282532992No Hit
GTTCCAATGGGGGGAGGTATCTTTCGATCCTGACTGGCACTGCCTCCTTT288780.14854336151394687No Hit
GTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAGAT288000.14814214320942135No Hit
CCTCTAATGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAA285220.14671216002149706No Hit
ATTCCCACCTGTACCACCACCTCTGGGTTCTCCATTTATTTTATTTTTAT284770.14648068792273233No Hit
ATCTTGAATAAGTGCATTGATAAGTTGAACTGCATATCTTGTTGATTCGG282740.1454364915660826No Hit
GCCTTGGAGTTGTCGGGGTGCACATTTTCCAAGTGTCCAAGTACAGAGTT282630.14537990949749566No Hit
ACCAAAAGGAGGCAGTGCCAGTCAGGATCGAAAGATACCTCCCCCCATTG281280.1446854932012015No Hit
ATGTTAGAGGGGGCTACCATTCCAGAATCATCTCTCTCCATTGGTTGATG280580.14432542549201194No Hit
TTCCTGGGTAACTCTGTACTTGGACACTTGGAAAATGTGCACCCCGACAA279010.14351784505854392No Hit
CCTCAAACTCATCCACCTCTGCCCCACCAACTTTGGGCCAACCAAAAGGA276180.14206214274853468No Hit
CATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTTTACCA274950.14142945234524443No Hit
GTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATT274450.14117226112439477No Hit
ACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTTAGCTGAGGTGATGG273830.14085334401054114No Hit
GTGTCCAACAGGAGCAACAAAACTGGTCCCCACAGGGGCTTGTGCGACAG267900.1378030561312638No Hit
GTTCCCTATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGT267370.13753043343716315No Hit
CCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCAC267040.13736068723140235No Hit
CTGTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAA266170.1369131745071239No Hit
CCTTTAGAAAAATCCAGAAAACTGCTTGCCATAGGTTGATGAAAAATGTT264150.13587412197489115No Hit
TGATAGGAGATACCCGTCACTGCTACTCCATTCCCAAGAAGAAAAGACAA263320.13544718454828064No Hit
GGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTG263180.13537517100644275No Hit
ATTCAAGATCCTGCTAAGGAACTGGAAGACTTGATTCCTAAAAATCATAT262750.135153986556512No Hit
TTCCAGGATAATGGGAAGGGGAGGGTGCAACATCACTGCTATACAAGACG261530.13452643997763877No Hit
TTCCAGAATCATCTCTCTCCATTGGTTGATGCTGGGAGAATGTGCTTGGG261190.13435154994746099No Hit
ACCCAGGAACGCTGAATTTGCTGGAACGTTCTTGTTAAGTTTATTAGTTG256090.13172819949479414No Hit
TCCTAGTGCAACAACCACCACCACAATCGGAATTTCTGCAACATCTGCAA255910.13163561065528825No Hit
GAACAACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAA255680.13151730269369738No Hit
CCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCCAGAAAACTGCT255170.1312549676484307No Hit
ATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTCCCAGCATCAA254300.13080745492415224No Hit
GTGCTAAGGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGG253690.13049368163471564No Hit
TCTTTGACCTTCGTACAACTTCTTTCCACCCTTCTTCTCTTTTCTGTCCC252170.12971182032333256No Hit
GCATTGGATGGTTCCCTATCACAGACTGTGACCAACCTGATAAGCCTTCT247810.12746911287752327No Hit
CTTGGAAAATGTGCACCCCGACAACTCCAAGGCACCTGGCTTCAGACCAC247130.1271193328171677No Hit
GATTTGGTGCTGGCAGGTGTTCTGATATGATTTTTAGGAATCAAGTCTTC246020.1265483683068814No Hit
AAACAAAGGAGACCCCTCCCACTGCACATTTAATATTACCAGAACAACAT243420.125210973958463No Hit
GTCAGAAGGCTTATCAGGTTGGTCACAGTCTGTGATAGGGAACCATCCAA242710.12484576242485644No Hit
GTCCAAGTTATCCGAGTCACTATTCCCACCTGTACCACCACCTCTGGGTT242650.12481489947835447No Hit
CTCCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCT242640.12480975565393748No Hit
CATTTAATATTACCAGAACAACATATATCCTTAGCACAGCAAAAAGCTGA241480.1242130720215662No Hit
CCACAGGGGCTTGTGCGACAGATCCTCCTTGCCGAATCCGGGCTAGCCTC240570.12374498399961976No Hit
CCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTT240480.12369868957986684No Hit
TTCTAATTCCTTATCAACAAGCTTTAAGTCAGTGCCATTGCCATTAAGTT240270.12359066926710995No Hit
CGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAAGAACGGCG239960.12343121071018315No Hit
CCCTATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGA239810.12335405334392824No Hit
GCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGA239160.12301970475682364No Hit
CTCCCAGCATCAACCAATGGAGAGAGATGATTCTGGAATGGTAGCCCCCT238410.1226339179255491No Hit
AGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTACCACCA237400.12211439165943275No Hit
GTTTATTAGTTGGCTGGAACGTTGATAATGTTGTCGCCTGTGATGTTACG235970.12137882476780262No Hit
GTACTTGGACACTTGGAAAATGTGCACCCCGACAACTCCAAGGCACCTGG235300.12103418853186404No Hit
GTATGGAGGCACCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAG232650.11967107506136068No Hit
GTACTGCTACTTCCAAAACTCAAACACGACTTGAAGGTGAAACGAATTCT231420.11903838465807043No Hit
CCCCTCCCACTGCACATTTAATATTACCAGAACAACATATATCCTTAGCA228030.11729462818070954No Hit
GGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAATGCACTTA227600.11707344373077881No Hit
GCAGGGGGGCGTCCGAGCAATGGGTGAGTTCTTGCACTCCAGCAGGAACT226380.11644589715190556No Hit
GGTCAAAGAAGTTGTCCGTTCCAGCTTCCGTGGTTTCCAGGATAATGGGA224670.11556630317659962No Hit
CCTCTGCCCCACCAACTTTGGGCCAACCAAAAGGAGGCAGTGCCAGTCAG224550.1155045772835957No Hit
CTAATAAACTTAACAAGAACGTTCCAGCAAATTCAGCGTTCCTGGGTAAC224190.11531939960458394No Hit
GTACAGGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAAC221650.1140128682026675No Hit
GTGCCAGTCAGGATCGAAAGATACCTCCCCCCATTGGAACAGAGAGGCTA221490.1139305670119956No Hit
GTTTAATGGACTTCACAACCCAGATCCTGCTTGGAACCCTATGATAAAAG218710.11250058382407133No Hit
GAAAAGAGAAGAAGGGTGGAAAGAAGTTGTACGAAGGTCAAAGAAGTTGT218700.11249543999965433No Hit
GTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGAATTCCTCT218480.11238227586248047No Hit
CTCTAACATTTTTCATCAACCTATGGCAAGCAGTTTTCTGGATTTTTCTA215960.11108603210939803No Hit
GGCCAGCTCCTGTTGGGACTCCAAGTTTCAACAGGCAACATTTTTCCCCC213000.10956346008196788No Hit
GAATTAGAATTCGTTTCACCTTCAAGTCGTGTTTGAGTTTTGGAAGTAGC212600.10935770710528811No Hit
CCATAGAACCTCCTAGTGCAACAACCACCACCACAATCGGAATTTCTGCA209370.10769625181859911No Hit
GAATCTAACTAGCCCCAAAAGGGGACAGAAAAGAGAAGAAGGGTGGAAAG208760.10738247852916251No Hit
CATCAATATGCGCACCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCT206530.10623540568417288No Hit
GCATATCTTGTTGATTCGGTACCACCCCTTATGGTGATCATTCTCTCGCC206400.10616853596675196No Hit
GCCATTAAGTTCTCCAAACATAAAGCTGAATCTAACTAGCCCCAAAAGGG205710.1058136120819794No Hit
CCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTTAGCTGAGGTGATGGG205240.10557185233438068No Hit
GATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGCA204250.1050626137170983No Hit
GCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGC203450.1046511077637388No Hit
TTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCA203000.10441963566497409No Hit
ATTCTGGAATGGTAGCCCCCTCTAACATTTTTCATCAACCTATGGCAAGC199840.10279418714920403No Hit
GTGGGAGGGGTCTCCTTTGTTTTGTTCTTCTTATTTTTCCGATTGGTGCT198890.10230552382958961No Hit
GGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTGGAACATCAGC198810.10226437323425368No Hit
CTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACA197390.10153395016704056No Hit
GAAGAGGAAGATGGGGAGCTGCCTGATGGATCAATGGATGGTAAGCCAAC196780.10122017687760393No Hit
CCATTGATCCATCAGGCAGCTCCCCATCTTCCTCTTCTGCTCCTCTGACA194890.1002479940627921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)