FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4349317
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG383420.8815637029906075No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC327550.7531067521636156No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA284250.6535508908640139No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC278520.6403764085257524No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC268970.6184189379619834No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG247460.5689628969330127No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG222840.5123563078984585No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA206680.4752010488083531No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA200950.46202656647009177No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC171830.39507352533742657No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC165370.38022061854769384No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT158970.3655056644526026No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA158950.3654596802210554No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA157810.3628385790228673No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT136590.31404930935133035No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG124620.286527746770355No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA122210.28098664686892216No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC120720.27756082161865875No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG119720.27526161004130073No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG114950.264294370817303No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT113130.2601098057465115No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG112380.25838539706349295No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA109210.2510968963632681No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA108420.24928051921715524No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA107970.24824587400734413No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG105200.24187705793806244No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA104260.2397157990553459No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA102540.23576115514229015No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA102500.2356691866791958No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG102380.23539328128991285No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA100360.23074887390364968No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT99580.22895548887331046No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC99060.22775989885308429No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG97460.22408116032931147No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC95700.22003454795316138No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA93400.21474636132523794No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT90790.20874541910833355No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA90730.20860746641369207No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC90680.20849250583482418No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT84710.19476621271799688No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA80380.1848106265880367No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT79790.18345409175739547No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT79780.18343109964162188No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA78810.18120086441158464No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT78560.1806260615172451No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT78060.1794764557285661No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA76160.17510795373158589No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG75100.17267078945958642No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG74350.1709463807765679No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT72360.16637094973762548No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA71850.16519835183317289No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA71070.16340496680283365No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC70340.1617265423513623No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGACCCCTCACTT66440.15275961719966605No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC66310.1524607196946095No Hit
GTTTATAGCACCCTTGGGTGTTTGACAAGTTGTATTGCAATCGTGGACTG63940.14701158825627103No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG63890.14689662767740314No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG63660.1463678090146108No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC63450.14588497458336563No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC62830.14445946340540364No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA62790.14436749494230933No Hit
GGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTG62580.14388466051106413No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC61130.14055080372389503No Hit
AGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTG60790.13976907178759332No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA60360.13878041080932937No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA60050.1380676552203484No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT59150.1359983648007262No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC58660.13487175112782077No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAACCCTTTTACTCCATTTGC58430.13434293246502843No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC58000.1333542714867645No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT57750.13277946859242495No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA57670.13259553166623633No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA57380.1319287603088025No Hit
GTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGG57340.13183679184570818No Hit
GTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCA56720.13041128066774624No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG56640.1302273437415576No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC56590.1301123831626897No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT56350.12956057238412377No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG56330.1295145881525766No Hit
ATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAAT56140.12907773795287858No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC55970.1286868719847277No Hit
GGCTCGAATCGACCGTGGGTGTCTTTCAACCAGAATCTGGAATATCAGAT55820.128341990248124No Hit
GGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA55810.12831899813235043No Hit
GTAATAGTTCATTCTCCCTTCTTGACCCCTCACTTTGGGTCTTATTGCTA55620.1278821479326524No Hit
GTCTGAACTAGGTGTTTCCACAATGTAGGACCATGAGCTTGCTGTGGAGA55420.12742230561718082No Hit
GTCCACGATTGCAATACAACTTGTCAAACACCCAAGGGTGCTATAAACAC55290.12712340811212428No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA55270.1270774238805771No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA55270.1270774238805771No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC55040.12654860521778477No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA54860.12613474713386033No Hit
ATCTTGATGACCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT54710.12578986539725664No Hit
CTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTG54530.1253760073133322No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT54470.1252380546186907No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA53650.12335270112525715No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT53300.12254797707318185No Hit
GGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCA53160.12222608745235172No Hit
CCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTGTCTGAACTA52780.12135238705295566No Hit
GGGTAACACGTTCCATTGTCTGAACTAGGTGTTTCCACAATGTAGGACCA52060.11969695471725791No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT51610.1186623095074468No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT51110.1175127037187678No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG51020.11730577467680557No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA50230.11548939753069276No Hit
TTATAGCACCCTTGGGTGTTTGACAAGTTGTATTGCAATCGTGGACTGGT49460.11371900461612709No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG49390.11355805980571203No Hit
GCTATAAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGG49360.11348908345839127No Hit
AATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCTGGCCAAGACCA49190.11309821749024042No Hit
CTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTT48950.1125464067116745No Hit
GTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGA48620.11178766689114636No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA48270.11098294283907106No Hit
GTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTG48110.11061506898669377No Hit
GTACCGAGATATGCATTCGCAATGGAAAGAAATGCTGGATCTGGTATTAT47850.11001727397658069No Hit
CAATTGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCC47800.1099023133977128No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA47650.10955743166110908No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT47620.10948845531378835No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT47580.10939648685069402No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC47290.1087297154932602No Hit
CCTCTAGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAA47170.10845381010397724No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG46830.10767207816767552No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT46700.10737318066261897No Hit
AGTCCAGATTGTGTTCTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGA46610.10716625162065677No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA46500.10691333834714739No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT46120.10603963794775133No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC46060.10590168525310985No Hit
GGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT45920.10557979563227975No Hit
AATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGG45810.10532688235877034No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT45670.10500499273794024No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC45430.10445318195937431No Hit
AAATAGGCCTCTAGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCA45150.10380940271771408No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA45060.10360247367575184No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT44900.10323459982337457No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA44610.10256782846594076No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA44600.10254483635016717No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT44450.10219995461356346No Hit
GAGTAAAAGGGTTTTCATTCAAATACGGCAATGGTGTTTGGATAGGGAGA44410.10210798615046913No Hit
CTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGACCCCTCACTTTG44330.1019240492242805No Hit
ATCAGATAGGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCT44250.10174011229809186No Hit
ACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTG44240.10171712018231828No Hit
TTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTG44130.1014642069088089No Hit
GATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAATGTTACATTTACC43990.10114231728797878No Hit
GGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTG43910.10095838036179015No Hit
GGGATAATTGGCATGGCTCGAATCGACCGTGGGTGTCTTTCAACCAGAAT43900.10093538824601657No Hit
GGGCTACCCCTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGA43740.10056751439363927No Hit
GTCCAGTAATAGTTCATTCTCCCTTCTTGACCCCTCACTTTGGGTCTTAT43630.1003146011201299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)