FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT90.2517482517482518No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC90.2517482517482518No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC80.22377622377622378No Hit
CCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCA80.22377622377622378No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG80.22377622377622378No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT80.22377622377622378No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA70.1958041958041958No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA70.1958041958041958No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC70.1958041958041958No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA70.1958041958041958No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT70.1958041958041958No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT60.16783216783216784No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA60.16783216783216784No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA60.16783216783216784No Hit
GATAAGGGGCGTCTGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGC60.16783216783216784No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG60.16783216783216784No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC60.16783216783216784No Hit
CTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAG60.16783216783216784No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC60.16783216783216784No Hit
GAAATAGATGGGGTAAAACTGGAATCAACAAGGATTTACCAGATTTTGGC50.13986013986013987No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA50.13986013986013987No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG50.13986013986013987No Hit
CTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTT50.13986013986013987No Hit
ATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTC50.13986013986013987No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC50.13986013986013987No Hit
GAAAGAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCC50.13986013986013987No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA50.13986013986013987No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC50.13986013986013987No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC40.11188811188811189No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA40.11188811188811189No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.11188811188811189No Hit
GAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCC40.11188811188811189No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA40.11188811188811189No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA40.11188811188811189No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA40.11188811188811189No Hit
ACTCTGTACTTGGACACTTGGAAAATGTGCACCCCGACAACTCCAAGGCA40.11188811188811189No Hit
TCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGA40.11188811188811189No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG40.11188811188811189No Hit
GGTATCTCCTATCATTAGAGGAATTCACATCCATAGTGAAATTTAACTCT40.11188811188811189No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG40.11188811188811189No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.11188811188811189No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA40.11188811188811189No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC40.11188811188811189No Hit
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT40.11188811188811189No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG40.11188811188811189No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT40.11188811188811189No Hit
GAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAG40.11188811188811189No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG40.11188811188811189No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT40.11188811188811189No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT40.11188811188811189No Hit
GGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACC40.11188811188811189No Hit
CCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGG40.11188811188811189No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG40.11188811188811189No Hit
AAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCT40.11188811188811189No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC40.11188811188811189No Hit
GTGCTAAGGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGG40.11188811188811189No Hit
GCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCA40.11188811188811189No Hit
GACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCA40.11188811188811189No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT40.11188811188811189No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG40.11188811188811189No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.11188811188811189No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG40.11188811188811189No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC40.11188811188811189No Hit
ATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCC40.11188811188811189No Hit
CCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCA40.11188811188811189No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.11188811188811189No Hit
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC40.11188811188811189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)