FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_E1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC140.2635046113306983No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC110.2070393374741201No Hit
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT110.2070393374741201No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG110.2070393374741201No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC110.2070393374741201No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG100.18821757952192736No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT90.16939582156973462No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA90.16939582156973462No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA80.1505740636175419No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC80.1505740636175419No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC80.1505740636175419No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT80.1505740636175419No Hit
GAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGC70.13175230566534915No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT70.13175230566534915No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA70.13175230566534915No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC70.13175230566534915No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT70.13175230566534915No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA70.13175230566534915No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG70.13175230566534915No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG70.13175230566534915No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA60.1129305477131564No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT60.1129305477131564No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC60.1129305477131564No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA60.1129305477131564No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG60.1129305477131564No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG60.1129305477131564No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA60.1129305477131564No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT60.1129305477131564No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC60.1129305477131564No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG60.1129305477131564No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT60.1129305477131564No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT60.1129305477131564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)