FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_D2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_D2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334618
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT138350.3191746077739722No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG124980.2883299058879006No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC118930.27437250525882556No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT118300.272919089986707No Hit
GTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAACAAAGGCAGAA101060.23314626571476427No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC98810.2279554968857694No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC91480.21104512554508842No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG89750.20705400106768349No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC88740.20472392261555689No Hit
CTTCAGGACCCCCCGGTTGGGGGGAACGTGCCCAGTAAAGAACTGAAATG85800.19794131801233694No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT83710.19311967052229284No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT83570.19279668935071095No Hit
TTCCTGAGCCCAGCAGGTGTCCTGGCTCTATTCTCTACGCATTGGGAAAT78480.18105401675534036No Hit
ATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAA77280.17828560671320978No Hit
TATAAAAACCACTCAAGCTTTAGGTACTGCTATTAATGCTAGAAATAACT66750.15399280859351389No Hit
CTTTAAGCCTGTGTCTCATTGAGCAAAAGAAGAGTCTACTTTCATAGCAC64930.14979405336294915No Hit
GGCTTAAAGATATGAAATGGAGTTATTTAATCCTCCCTCCTCAAAGTGCA64460.14870975942978135No Hit
GTGTAAGGGCTCTTTATTTCTCATTGCGATGATGTACTCGTTAGGAATAA62200.14349592051710208No Hit
CATGTAAATAATGGCTAATTTCAAATACACCTGACCCTTTTGGAAATGTT61550.14199636507761468No Hit
CCATAGTGCTTTCTTGTAAAATCTGAGAAAGAATATCTAAGAATCCATTT58970.13604428348703393No Hit
CCCAAAAGGTGTGCCACAGGGAGGATCGGGGTGGATAGTCTGGCATCAAC58460.13486770921912844No Hit
AATGTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGG58060.13394490587175156No Hit
GTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGGAAA57300.13219157951173552No Hit
ATTCTCTACGCATTGGGAAATGCGAAATAGGCCCGCAGAGCCACTTCAGG56250.12976922072487126No Hit
ACTTAATACTTAAAACTCTAAATCTTGAGATGGTAGAATAGCTAACCAAA55840.12882334729380995No Hit
ATCCAGAACATTCTGCAAGTTTGTCCTGATCTAAAATAACAACAATGCTG55760.12863878662433462No Hit
GGCTATTAGTTGTCCAAATGGAATAATCCCCTTAAGCCTTTTCAAGAAAA55730.12856957637328134No Hit
CTATGGAATCTTGTAGACAAAGAGAAAATTTTTTTAAGCTTGCAGCTTGG55720.1285465062895969No Hit
GTATAGGAATAAAGCACTCTCATTAATCAAATATTTTATCTTTTGCTTTC55220.12739300210537582No Hit
TCTTCATACTTAGAAAGTGCTATGAAAGTAGACTCTTCTTTTGCTCAATG53250.1228481956195448No Hit
CAATTTATACTGTATAGTTTGCAGCCTTTTAGTTACAAAAGGTCTGTTTT52840.1219023221884835No Hit
TGGATGGACTGAGCATTCCTGAGCCCAGCAGGTGTCCTGGCTCTATTCTC52660.12148706068216392No Hit
GTATGATGGCTTATTGATGAATAAACTTTGTAACATTTTCATTCTTGTCA52400.12088723850636897No Hit
GTATAAGCCATTAGAAATGAAATTACAGAGCTATAGGAAGGGAACAAGGT51840.11959531382004136No Hit
TACTTAGAAAGTGCTATGAAAGTAGACTCTTCTTTTGCTCAATGAGACAC51740.11936461298319714No Hit
GCACTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAA51280.11830338913371373No Hit
GAATAGTTAATGTCAAAGTATTCTCAGGAAAGGAAAGAAAGAGAAAAGCT50640.11682690377791077No Hit
GTTCTATGCTCTCCAGGAAAGCAGTGGGATCTAATAAAATGTTGGTCCTC50390.11625015168580022No Hit
CCTTATAACAGTACAATGTTACTGCCACACACATCAGTTTTCTTGCCTAA50280.11599638076527159No Hit
ACCCAGGTGCTCCCAAGCTGCAAGCTTAAAAAAATTTTCTCTTTGTCTAC49830.11495822699947261No Hit
CACTAAAGCTGTCTGAATTCATGTATCTTCATGTATATCCATGGAACACA48870.11274349896576814No Hit
ATTTACATGGGTGCACTGCCTTTAACCCGGGCTTCCAAAGGTGTTGGTTC48090.11094403243838327No Hit
GTAGAATAGCTAACCAAAATTCTGACCTCCATAGTGCTTTCTTGTAAAAT46690.10771422072256426No Hit
ATCATGACCTGAGCCGAAAAGGCAGACGCTTAACCAATTGAGCCACCCAG46520.10732202929992908No Hit
GTGTTAGGTTGATTAAAAAAATGTTTTATCAATAAAAACTGTCCAAAAGC46040.10621466528307685No Hit
ATCCAAAAGAGGTAAGCTATTAAATCTTGACTCTAAAGGTTTATTTTTTT44940.10367695607779047No Hit
GTATAGTGCCTGCTGGAGACCCAAAACCTGTTCTGGGGAAGGCCATGATG44520.10270801256304478No Hit
TCTTAAATCCTTGCCATTAAAATGTAAGGGCATTAGTACTAATAGAACAA44310.1022235408056719No Hit
GTATAGTTTGCAGCCTTTTAGTTACAAAAGGTCTGTTTTTCTTAGATTTT43970.1014391579604016No Hit
GGATTAAATAACTCCATTTCATATCTTTAAGCCTGTGTCTCATTGAGCAA43960.10141608787671717No Hit
CCATTATCCCTGGCCACCAGCTCCCCCAGCCTCTTTTTACTTTGTTCTTA43820.10109310670513526No Hit
ATTTAATCCTCCCTCCTCAAAGTGCAAAGAGTTCGAGAACTAGAATTGCA43560.10049328452934031No Hit
CCCCTATGCCGGGATATCAAGGGTCATATTCGTTTTTGGTTTTTTGGACT43530.10042407427828703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)