FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_D10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_D10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9409
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTC2112.2425337442873845No Hit
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAG1871.987458816027208No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAA1791.9024338399404823No Hit
AGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGG1331.413540227441811No Hit
AGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTA1171.24349027526836No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCT1131.2009777872249974No Hit
AACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCA971.0309278350515463No Hit
GGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGA920.9777872249973429No Hit
GAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACT890.945902858964821No Hit
GTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGAC860.914018492932299No Hit
GAGCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTG760.807737272823892No Hit
AAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAA700.7439685407588479No Hit
GGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGA680.7227122967371665No Hit
GTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGC650.6908279307046444No Hit
CAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACA620.6589435646721225No Hit
GCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAAC560.5951748326070783No Hit
CTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTG560.5951748326070783No Hit
CACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCT450.47826549048783074No Hit
AGCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGG410.4357530024444681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410.4357530024444681No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA400.4251248804336274No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACCTGTCT390.41449675842278666No Hit
AGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAA380.403868636411946No Hit
TGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTC360.3826123923902647No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTGTCTCTTA350.37198427037942394No Hit
GGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGC320.3400999043469019No Hit
ATTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTC290.30821553831437987No Hit
GGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAG290.30821553831437987No Hit
GAGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTG270.2869592942926985No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT260.27633117228185783No Hit
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCT260.27633117228185783No Hit
GAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCA260.27633117228185783No Hit
GCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGG250.2657030502710171No Hit
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTCTCTTA250.2657030502710171No Hit
GTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGAC240.2550749282601764No Hit
AAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCT240.2550749282601764No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGTCTTGGCTGTTTTGCCCA240.2550749282601764No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCC230.24444680624933574No Hit
GCAAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGG210.22319056222765438No Hit
GTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCGCTCTGTCTCT200.2125624402168137No Hit
GAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTT200.2125624402168137No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCT190.201934318205973No Hit
TTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCC180.19130619619513234No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCTGTCTCTTATA180.19130619619513234No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCCTGTCTCTT180.19130619619513234No Hit
GCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC180.19130619619513234No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTCTGTCTCTTAT180.19130619619513234No Hit
GTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCTGTCTCTTATA180.19130619619513234No Hit
GGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCTGTCTCT160.17004995217345095No Hit
GAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCA160.17004995217345095No Hit
GGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCTGTCTC160.17004995217345095No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTCG150.15942183016261027No Hit
TAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCT150.15942183016261027No Hit
AACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCTG130.13816558614092891No Hit
AAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCCT130.13816558614092891No Hit
AGCGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCTGTCTCT130.13816558614092891No Hit
AGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACCTGTCTCTTAT130.13816558614092891No Hit
TATTAGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGT130.13816558614092891No Hit
GTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTCTG130.13816558614092891No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT120.1275374641300882No Hit
TCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACC120.1275374641300882No Hit
AGTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGCTGTC120.1275374641300882No Hit
CTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACTCTGTC110.11690934211924754No Hit
GTAGAAACAAGGTCTTGGCTGTTTTGCCCAGGCTGGTCTCTGTCTCTTAT110.11690934211924754No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT110.11690934211924754No Hit
GTCTTGGCTGTTTTGCCCAGGCTGGTCTTGAAGTCCTAGACCTGTCTCTT110.11690934211924754No Hit
TGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATG110.11690934211924754No Hit
CGAAAGCAGGGAGGGTCACTTGAGTCTAGGACTTCAAGACCAGCCTGGGC100.10628122010840685No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTCTG100.10628122010840685No Hit
GCCCAGGCTGGTCTTGAAGTCCTAGACTCAAGTGACCCTCCCTGCTTTTG100.10628122010840685No Hit
ACTTGAGTCTAGGACTTCAAGACCAGCCTGGGCAAAACAGCCAAGACCTT100.10628122010840685No Hit
AGACCAGCCTGGGCAAAACAGCCAAGACCTTGTTTCTACTCTGTCTCTTA100.10628122010840685No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT100.10628122010840685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)