FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_C7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_C7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377399
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC43821.161105355340104No Hit
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT43771.1597804975635866No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG42421.1240093375976088No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC34880.924220784898741No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT28790.7628531077188864No Hit
CTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCG26640.7058842233286257No Hit
GGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAG23640.6263927567375642No Hit
CACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATT23170.613939093638298No Hit
TAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACT22170.5874419381079441No Hit
AATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTA20950.5551154083609124No Hit
ATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCA20610.5461063754805922No Hit
ATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTC17780.47111942532969087No Hit
GTATAACACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAA15870.42050985826671505No Hit
CTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACG15320.4059364227250205No Hit
ACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGA15120.40063699161894967No Hit
GTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGTTG14570.3860635560772551No Hit
GTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTT14470.38341384052421973No Hit
CATTAATACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGA12860.3407534201203501No Hit
ACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCATACGTAA12810.3394285623438324No Hit
GTATAGAGCAGGCACGACTTGCACGGGCATATTCACGAGTCGTTCATGAA12240.3243251836915307No Hit
ATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCTCTATAAAGAAG12200.32326529747031657No Hit
GCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAA11730.31081163437105025No Hit
CATTTACACACATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTT11610.3076319757074078No Hit
GTCTATTTCCATACAACCGATACGTATTTACCATACGCAAGAGTATTCAA11590.30710203259680074No Hit
CCATACAACCGATACGTATTTACCATACGCAAGAGTATTCAACGCAGAGA11430.30286248771194413No Hit
ACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGT11250.29809299971648046No Hit
GAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGG11040.29252859705510614No Hit
GCTCTATACCGCACGCTGGATCAAGAAATGCAAGCGGCGGTGAGCCTCTA10890.28855402372555305No Hit
CCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCGTG10750.28484442195130355No Hit
GAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAA10290.27265573040734076No Hit
GTGTTATACAAATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATA10230.27106590107551953No Hit
ACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGT10140.2686811570777877No Hit
GAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAG10130.26841618552248414No Hit
GTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTG9690.25675743708912846No Hit
CATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTC9440.25013314820654003No Hit
TCTTTATAGAGCGATGTGTATTTATTTGTATAACACAAACACCTACATCT9260.24536366021107636No Hit
TTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAA9220.2443037739898622No Hit
GGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGG9120.24165405843682683No Hit
ATGCAGGACAGCTTGTAGCATCCTCCAGTCGACCAGCAGCGGCAGCCGAA8650.22920039533756048No Hit
GAGATACACGTTGTCGTTGTTTGCATATAAGCGTACAGAAACGTTTACAT8610.22814050911634637No Hit
GGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACA8510.22549079356331098No Hit
TCCTAATAGTATGGCAATCGACAACGCCCCTTCTTCCGATACCTTCCTCT8420.2231060495655791No Hit
AAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAA8400.22257610645497208No Hit
ATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCAAGA8390.2223111348996685No Hit
CTATTAGGATCTGAGGTGGTGGAGGGCTTCTTGGACTTCTTCTTCTTCTT8370.22178119178906142No Hit
GTGTAAGATGGAGGAATAGACATAATCCAGCGAGAAGAAGGAGGGGTTTC8340.22098627712315083No Hit
GAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGA8290.21966141934663314No Hit
ACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTGTTTGCATATAA8250.21860153312541897No Hit
GAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAG8230.2180715900148119No Hit
GAGATAACTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTT8140.21568684601708005No Hit
AAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTCAACAG8110.21489193135116944No Hit
CTTCTTTATAGAGCGATGTGTATTTATTTGTATAACACAAACACCTACAT8070.2138320451299553No Hit
CGTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTT7960.2109173580216164No Hit
CACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATA7920.20985747180040223No Hit
TCTGTATTCTGTGTTCGCAGCTGTTCTCTGAGGATCGTAGGCCAAGAGTG7640.20243826825190317No Hit
GTGTGAGAGTAGCTGCGTAGTCAAGTTCTTTACAGCTTGATAGTCTGATT7490.19846369492235008No Hit
CCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAG7460.19766878025643947No Hit
AATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTT7380.1955490078140112No Hit
GTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTTTCTTCCTCTC7300.19342923537158285No Hit
TCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATA7240.19183940603976163No Hit
AACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAAT7180.1902495767079404No Hit
AAGTTATACCGCTCGGTAGCAATACAGGTATCGCAGGTGCGTCAAATCAG7150.1894546620420298No Hit
GAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAA7110.18839477582081565No Hit
AGCGAAAGCAGGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAA6960.18442020249126256No Hit
CTGTATGATAGATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCT6910.18309534471474487No Hit
CTCTTGCGTATGGTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTA6650.1762060842768529No Hit
CTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCATACGT6630.1756761411662458No Hit
GGCCAAGAGTGCGCCGAAGTCGGTTGTCGAGCTAGAGGCAGGAGGGACTT6460.17117162472608566No Hit
CTTATATGTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGA6360.16852190917305027No Hit
CCTACATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTA6330.16772699450713968No Hit
GTCCTGCATCGAGCTTGACTGCTTTCGTCGTAGAGGGTTGCATGCTTTCA6260.16587219362001487No Hit
GCATATAAGCGTACAGAAACGTTTACATTAATACATATAAGTAAACGCGT6250.16560722206471137No Hit
GTATGATAGATGTAGGTGTTTGTGTTATACAAATAAATACACATCGCTCT6220.16481230739880073No Hit
GTATAACTTCCCGCTCATTCGCACTGAGACCATTTCCTCTGAGTGTATAT6180.1637524211775866No Hit
CTTCTACGCTGTCACAATCATCTTGAAGCGACGAGTGAAGCTTCCCTCCA6150.16295750651167598No Hit
CAGGAAAGGAAGAGAGGAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAA6100.16163264873515829No Hit
GAATACTCTTGCGTATGGTAAATACGTATCGGTTGTATGGAAATAGACTT6060.16057276251394412No Hit
CTCCATAATAGTACCAAGAAGAGATTGATGATTGATAGTGTAAGATGGAG6040.16004281940333703No Hit
ATGCAAAGGAGTTAGATGTAGCGGGCATAGCGGATCATCTGATAGAGCAG5940.15739310385030167No Hit
AGAGTATTCAACGCAGAGATACACGTTGTCGTTGTTTGCATATAAGCGTA5880.15580327451848044No Hit
AGCGTACGTAGTTATCTCTGTTTTCTTTCTTCCTCTCCTTCGTGTTTTTC5790.1534185305207486No Hit
GAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAA5770.15288858741014152No Hit
GGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAA5720.15156372963362383No Hit
ACCGATACGTATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGT5710.1512987580783203No Hit
ATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATGAGTCA5670.15023887185710616No Hit
GCTATATACTCAAGTAGCGTGTAGGTAAACGCACGGATGTCCACGGCAGC5580.1478541278593743No Hit
GAAGAAAAACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAAC5520.14626429852755307No Hit
CCTTTGCATCGTGTGAGAGTAGCTGCGTAGTCAAGTTCTTTACAGCTTGA5520.14626429852755307No Hit
CTCTCACACGATGCAAAGGAGTTAGATGTAGCGGGCATAGCGGATCATCT5490.14546938386164246No Hit
GAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTAC5490.14546938386164246No Hit
GATAACTACGTACGCTCGTTCATTTACACACATAAATACGAGGCTTTTCA5390.14281966830860707No Hit
CTCCTGTGATGCGCCCACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCG5300.14043492431087523No Hit
CTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATAC5270.13964000964496462No Hit
AATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCT5270.13964000964496462No Hit
GATACACGTTGTCGTTGTTTGCATATAAGCGTACAGAAACGTTTACATTA5250.13911006653435753No Hit
ACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTC5240.13884509497905398No Hit
GTATTTATTTGTATAACACAAACACCTACATCTATCATACAGAAGTCTAT5210.1380501803131434No Hit
CTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGAC5170.13699029409192923No Hit
GTAGAAAGGTTTGCGATTAGAAAGCCTTCGCGTAGCTTGTCGGGCGGCCT5110.135400464760108No Hit
GAATACAGATATACACTCAGAGGAAATGGTCTCAGTGCGAATGAGCGGGA5070.13434057853889386No Hit
CAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCGTATGGTAAATAC5060.1340756069835903No Hit
TATTTACCATACGCAAGAGTATTCAACGCAGAGATACACGTTGTCGTTGT5000.13248577765176908No Hit
CACATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGA4960.13142589143055491No Hit
GTGCAGGGCCAAGCATTCTCATTCACTCATCTCCTCTTTATTTCCCGCGT4890.12957109054343016No Hit
TGCCCGGCCTGTTGACTCATTTCGCTTTTTTATTTCTTTCGTTTCCACGC4880.1293061189881266No Hit
ATAAAGACGTTTACTTTACGTATGAAAAGCCTCGTATTTATGTGTGTAAA4870.12904114743282308No Hit
CGTTTACTTTACGTATGAAAAGCCTCGTATTTATGTGTGTAAATGAACGA4860.12877617587751955No Hit
GAAAAACACGAAGGAGAGGAAGAAAGAAAACAGAGATAACTACGTACGCT4860.12877617587751955No Hit
AAAGCAGGCTGGTCTCCAACTCCCGACCTCCTGTGATGCGCCCACCTCGG4800.1271863465456983No Hit
ACACAAACACCTACATCTATCATACAGAAGTCTATTTCCATACAACCGAT4800.1271863465456983No Hit
ATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATCCCTTC4780.12665640343509124No Hit
ACTGTATGTCTGTCCTTGCCTGAGATCCTAGATTTCAGCGATCACAACTC4750.12586148876918063No Hit
GATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTTCGCTTTT4720.12506657410327002No Hit
TTCCTGAAGAGCAAACAAAGGACTACGCGCAGGCAGTTGCGATTGAAATT4710.12480160254796649No Hit
GGTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTATGATAGATGTA4700.12453663099266295No Hit
CATCTATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCAT4640.12294680166084172No Hit
CTCTTGGCCTACGATCCTCAGAGAACAGCTGCGAACACAGAATACAGATA4640.12294680166084172No Hit
GTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTC4620.12241685855023464No Hit
ATCAGGGGCAGCGCACGACGCGACTTCTATGTTTGGTCGTACGGCACGCG4610.1221518869949311No Hit
CTCAAGTAGCGTGTAGGTAAACGCACGGATGTCCACGGCAGCACGCCAAT4600.12188691543962756No Hit
GGTCTACACCCCATTCCAATCTATCTCTGACACTTCTTCCAGGAACAGGC4590.12162194388432401No Hit
GGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTGACTCATTTCGCTT4560.12082702921841341No Hit
GTAAATACGTATCGGTTGTATGGAAATAGACTTCTGTATGATAGATGTAG4560.12082702921841341No Hit
AAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTGTATCTCTGCG4540.12029708610780633No Hit
CACTGAAAGTGTGACGCCGCAGGACTTCGTCAACGTCGACCTCGACTTTT4500.11923719988659219No Hit
AAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACACACATAAATA4450.11791234211007448No Hit
GTAGCAATACAGGTATCGCAGGTGCGTCAAATCAGCCCGAACTGTCGGAG4440.11764737055477094No Hit
ATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTA4440.11764737055477094No Hit
CTCGTAGAGGGATAGAAGTAAGTCATGTACAAGCTACGCCGCCAGCATCT4410.11685245588886034No Hit
CTCTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCCGGCCTGTTG4400.1165874843335568No Hit
GATATACACTCAGAGGAAATGGTCTCAGTGCGAATGAGCGGGAAGTTATA4400.1165874843335568No Hit
TAGTTATCTCTGTTTTCTTTCTTCCTCTCCTTCGTGTTTTTCTTCCTTCC4390.11632251277825326No Hit
GTGTTTGTGTTATACAAATAAATACACATCGCTCTATAAAGAAGGGATCG4300.1139377687805214No Hit
GCCCTGCACACATCATCAGTCTTTCATTGTAGCTGAAACGGAAGTAGAGG4300.1139377687805214No Hit
GTGTATATCTGTATTCTGTGTTCGCAGCTGTTCTCTGAGGATCGTAGGCC4290.11367279722521786No Hit
GACGTTTACTTTACGTATGAAAAGCCTCGTATTTATGTGTGTAAATGAAC4290.11367279722521786No Hit
GCGTAGAAGTGTTGCATCTCTTCATTCGACTCAGCTAGGCACGGCGCAGA4270.11314285411461081No Hit
ACCGTGCCCGGCCTGTTGACTCATTTCGCTTTTTTATTTCTTTCGTTTCC4240.11234793944870018No Hit
TCTCTGCGTTGAATACTCTTGCGTATGGTAAATACGTATCGGTTGTATGG4220.1118179963380931No Hit
CACCTCGGCCTCTCGAAGTGCTGGGATGACGGGCGTGAGCCACCGTGCCC4210.11155302478278956No Hit
ATACAAATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGAC4200.11128805322748604No Hit
TCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGA4200.11128805322748604No Hit
GGAAGAAAGGAAGGAAGAAAAACACGAAGGAGAGGAAGAAAGAAAACAGA4180.11075811011687896No Hit
GTATATAGCTGAGAAAAAGGCGCCAGGACAAACCCTACATCGCTTCTCTA4110.10890330922975419No Hit
CGTTTACATTAATACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAA4030.10678353678732588No Hit
GGAAGAAAGAAAACAGAGATAACTACGTACGCTCGTTCATTTACACACAT4020.10651856523202234No Hit
AATACATATAAGTAAACGCGTGGAAACGAAAGAAATAAAAAAGCGAAATG4010.1062535936767188No Hit
ATCTGATAGAGCAGGAGGAAGAAGTGGGAAGCACAGTCAAATCTGATGGT3990.10572365056611172No Hit
ATCGTATGCTATCCTCCTGTACCTCCTATGGCATACACTGAAAGTGTGAC3970.10519370745550466No Hit
AACCTAAAGAAGGAGAGAAAGGAAGAAAGGAAGGAAGAAAAACACGAAGG3940.10439879278959403No Hit
GTACAGGAGGATAGCATACGATATCTGAGCCAGAATCAGAGTCCTCCTCA3930.10413382123429049No Hit
TATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCGTATG3900.10333890656837989No Hit
CTCCTGTACCTCCTATGGCATACACTGAAAGTGTGACGCCGCAGGACTTC3880.10280896345777281No Hit
GAAATAGACTTCTGTATGATAGATGTAGGTGTTTGTGTTATACAAATAAA3820.10121913412595158No Hit
TTCCTATCCGGCGCATTCGTATACTTGAATTCCATCCACCCATTATTCCC3820.10121913412595158No Hit
GCCTCGTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTT3800.1006891910153445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)