FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_C1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_C1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4477
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC120.2680366316729953No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC100.2233638597274961No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC100.2233638597274961No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG90.20102747375474647No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80.17869108778199688No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA80.17869108778199688No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC80.17869108778199688No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA70.15635470180924727No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA70.15635470180924727No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT70.15635470180924727No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT70.15635470180924727No Hit
CAATGGGGCAGTGGCTGTGTTAAAGTACAACGGCATAATAACAGACACTA60.13401831583649765No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT60.13401831583649765No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC60.13401831583649765No Hit
GAATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG60.13401831583649765No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT60.13401831583649765No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.13401831583649765No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA60.13401831583649765No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT60.13401831583649765No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT60.13401831583649765No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA60.13401831583649765No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT60.13401831583649765No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA60.13401831583649765No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA50.11168192986374804No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT50.11168192986374804No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA50.11168192986374804No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT50.11168192986374804No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG50.11168192986374804No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG50.11168192986374804No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT50.11168192986374804No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT50.11168192986374804No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG50.11168192986374804No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT50.11168192986374804No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC50.11168192986374804No Hit
ATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCT50.11168192986374804No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG50.11168192986374804No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC50.11168192986374804No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.11168192986374804No Hit
GCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC50.11168192986374804No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA50.11168192986374804No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT50.11168192986374804No Hit
GCTTTGAATTTTACCACAAATGCGATAACACGTGCATGGAAAGTGTCAAA50.11168192986374804No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.11168192986374804No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50.11168192986374804No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC50.11168192986374804No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT50.11168192986374804No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT50.11168192986374804No Hit
GTGTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGGGAAGGGTA50.11168192986374804No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT50.11168192986374804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)