FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762189
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC37090.21047685577426709No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT31340.17784698463104695No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA28200.16002823760674934No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27970.15872304276102053No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT25630.14544410389577964No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA24960.1416420145625696No Hit
CTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACC21060.11951044978716811No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC19660.11156578550881885No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT18860.10702597734976214No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG18630.10572078250403333No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18250.1035643736284814No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC17920.10169170276287048No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG17890.10152145995690587No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA17810.1010674791410002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)