FastQCFastQC Report
Fri 16 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA100.44943820224719105No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC90.40449438202247195No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG80.3595505617977528No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT80.3595505617977528No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT70.3146067415730337No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA70.3146067415730337No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA70.3146067415730337No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA60.2696629213483146No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT60.2696629213483146No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA60.2696629213483146No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT60.2696629213483146No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG50.22471910112359553No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC50.22471910112359553No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC50.22471910112359553No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG50.22471910112359553No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC50.22471910112359553No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG50.22471910112359553No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT50.22471910112359553No Hit
GATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTG50.22471910112359553No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG50.22471910112359553No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC40.1797752808988764No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT40.1797752808988764No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG40.1797752808988764No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC40.1797752808988764No Hit
TTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTC40.1797752808988764No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT40.1797752808988764No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT40.1797752808988764No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG40.1797752808988764No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC40.1797752808988764No Hit
AACTTATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA40.1797752808988764No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC40.1797752808988764No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG40.1797752808988764No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT40.1797752808988764No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA40.1797752808988764No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC40.1797752808988764No Hit
ATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAG40.1797752808988764No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT40.1797752808988764No Hit
GGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGAT40.1797752808988764No Hit
GATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGG40.1797752808988764No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA40.1797752808988764No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT40.1797752808988764No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT40.1797752808988764No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT40.1797752808988764No Hit
CTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGACCAGGA40.1797752808988764No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC30.1348314606741573No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA30.1348314606741573No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG30.1348314606741573No Hit
GAACTCAAACTCAGTGATTATGAGGGACGACTAATCCAGAATAGCATAAC30.1348314606741573No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA30.1348314606741573No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT30.1348314606741573No Hit
CACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTA30.1348314606741573No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA30.1348314606741573No Hit
CCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCT30.1348314606741573No Hit
AAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGT30.1348314606741573No Hit
ATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACC30.1348314606741573No Hit
GACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGG30.1348314606741573No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC30.1348314606741573No Hit
GGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGA30.1348314606741573No Hit
CTCCAACTCTTGATAGTGTCTGTTATTATGCCGTTGTACTTTAACACAGC30.1348314606741573No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC30.1348314606741573No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG30.1348314606741573No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC30.1348314606741573No Hit
CCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGC30.1348314606741573No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCAC30.1348314606741573No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT30.1348314606741573No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT30.1348314606741573No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG30.1348314606741573No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT30.1348314606741573No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG30.1348314606741573No Hit
GTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGACTCGAACA30.1348314606741573No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC30.1348314606741573No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT30.1348314606741573No Hit
CCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTT30.1348314606741573No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT30.1348314606741573No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT30.1348314606741573No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT30.1348314606741573No Hit
GATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTG30.1348314606741573No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT30.1348314606741573No Hit
AGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATACCTAG30.1348314606741573No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.1348314606741573No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT30.1348314606741573No Hit
GTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAA30.1348314606741573No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT30.1348314606741573No Hit
CTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATT30.1348314606741573No Hit
CTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCAT30.1348314606741573No Hit
CTCTATTGTTATGCTATTCTGGATTAGTCGTCCATCATAATCACTGAGTT30.1348314606741573No Hit
AATGTGCACTGAACTCAAACTCAGTGATTATGAGGGACGACTAATCCAGA30.1348314606741573No Hit
TTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCA30.1348314606741573No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG30.1348314606741573No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC30.1348314606741573No Hit
CACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATCCACTAATC30.1348314606741573No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA30.1348314606741573No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG30.1348314606741573No Hit
CTTTCCGTCTACTCTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCT30.1348314606741573No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA30.1348314606741573No Hit
TCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCA30.1348314606741573No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC30.1348314606741573No Hit
ACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAA30.1348314606741573No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG30.1348314606741573No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT30.1348314606741573No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30.1348314606741573No Hit
GGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACC30.1348314606741573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)