FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC80.38314176245210724No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC80.38314176245210724No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA80.38314176245210724No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC70.33524904214559387No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT60.28735632183908044No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA60.28735632183908044No Hit
GTGTTGGTCTCTGTGATCAGCATGCTCACCTCCAGGTCACAGTAGAGCAG60.28735632183908044No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA50.23946360153256704No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT50.23946360153256704No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA50.23946360153256704No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC50.23946360153256704No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA50.23946360153256704No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC50.23946360153256704No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC40.19157088122605362No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG40.19157088122605362No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA40.19157088122605362No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC40.19157088122605362No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT40.19157088122605362No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA40.19157088122605362No Hit
CTCTAGAGTGACTCAGTGGGGTAGGAGCTATTATTATCTTCATTGTCTAA40.19157088122605362No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA40.19157088122605362No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC40.19157088122605362No Hit
GGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT40.19157088122605362No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC40.19157088122605362No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG40.19157088122605362No Hit
CAATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATCTG40.19157088122605362No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCTCTTAG40.19157088122605362No Hit
CTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAA40.19157088122605362No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT40.19157088122605362No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC30.14367816091954022No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30.14367816091954022No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG30.14367816091954022No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG30.14367816091954022No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT30.14367816091954022No Hit
CCTCCAGGTCACAGTAGAGCAGGGGGCTTTGATAGGTGTGGGCCAGCTTC30.14367816091954022No Hit
CAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAA30.14367816091954022No Hit
GGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATCGCCAAA30.14367816091954022No Hit
TCCTTGTTGATTCCAGTTTTACCCCATCTATTTCTTCTCTGTTTAATTTT30.14367816091954022No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA30.14367816091954022No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT30.14367816091954022No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGAC30.14367816091954022No Hit
CTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCA30.14367816091954022No Hit
TGCTAGAGCCTGGCATATAAGATGCACTCCAGATGAAAGCACATCATCCT30.14367816091954022No Hit
GGTGTGAAGTGAGGATGATGTGCTTTCATCTGGAGTGCATCTTATATGCC30.14367816091954022No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT30.14367816091954022No Hit
AAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATAT30.14367816091954022No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG30.14367816091954022No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATCTGTC30.14367816091954022No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.14367816091954022No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT30.14367816091954022No Hit
CTTCCCCACTCTGGGGCTCAGTTTCTTTCTAGTCGCGAGGGTGACAGTCC30.14367816091954022No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30.14367816091954022No Hit
CTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTG30.14367816091954022No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT30.14367816091954022No Hit
CCAATGAACTGGCGACAGTTGAATAGATCGCCAAAATCTGGTAAATCCTT30.14367816091954022No Hit
CATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTT30.14367816091954022No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA30.14367816091954022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)