FastQCFastQC Report
Tue 6 Feb 2024
HVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVLTFDRX3_n02_CIVIR_Ferret_Flu_R2_B1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC190.5581668625146886No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT110.32314923619271446No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCT110.32314923619271446No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC110.32314923619271446No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT100.2937720329024677No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA90.26439482961222094No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC90.26439482961222094No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA80.23501762632197415No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA80.23501762632197415No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGCTCTTAG80.23501762632197415No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC70.2056404230317274No Hit
CTTCACACCTGTGTACCCCAAGGCTCAGGGTGACCTGCCCAGGTCATAGC70.2056404230317274No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC70.2056404230317274No Hit
AATTAGAGCACATCCAGAAACTGATTGCCCCCAGCTCTTAGTTTGCATAG60.17626321974148063No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA60.17626321974148063No Hit
CAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACA60.17626321974148063No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA60.17626321974148063No Hit
ACTCTAGAGTGACTCAGTGGGGTAGGAGCTATTATTATCTTCATTGTCTA50.14688601645123384No Hit
GTGTACCCCAAGGCTCAGGGTGACCTGCCCAGGTCATAGCCAGTGAGTAG50.14688601645123384No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG50.14688601645123384No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT50.14688601645123384No Hit
CCTCCAGGTCACAGTAGAGCAGGGGGCTTTGATAGGTGTGGGCCAGCTTC50.14688601645123384No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA50.14688601645123384No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT50.14688601645123384No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50.14688601645123384No Hit
ACTTATGACTACCCAAAATACTCAGAGGAAGCAAAATTAAACAGAGAAGA50.14688601645123384No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA50.14688601645123384No Hit
GCTCTACTGTGACCTGGAGGTGAGCATGCTGATCACAGAGACCAACACTA50.14688601645123384No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA50.14688601645123384No Hit
ATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAG50.14688601645123384No Hit
ATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTA40.11750881316098707No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40.11750881316098707No Hit
CTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATG40.11750881316098707No Hit
TCCAGAAACTGATTGCCCCCAGCTCTTAGTTTGCATAGTTTCCCGTTATG40.11750881316098707No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.11750881316098707No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT40.11750881316098707No Hit
GGCATATAAGATGCACTCCAGATGAAAGCACATCATCCTCACTTCACACC40.11750881316098707No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG40.11750881316098707No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG40.11750881316098707No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA40.11750881316098707No Hit
GTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACAGAGTGTGTTACTGTT40.11750881316098707No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA40.11750881316098707No Hit
CAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGTC40.11750881316098707No Hit
CCGGCACACCGTGGTGAAGCTGGCCCACACCTATCAAAGCCCCCTGCTCT40.11750881316098707No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT40.11750881316098707No Hit
GATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGG40.11750881316098707No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA40.11750881316098707No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT40.11750881316098707No Hit
GGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAA40.11750881316098707No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT40.11750881316098707No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40.11750881316098707No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40.11750881316098707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)